Anti-Phospho-MAP2K4 (S257) Rabbit Monoclonal Antibody for WB, IHC-P - P45985

Core Product Specifications and Parameters

Biological Background: MAP2K4 Function and Localization

• Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4), also known as JNKK1, MEK4, MKK4, SEK1, SKK1, SERK1, MAPKK4, PRKMK4, SAPKK1.
• Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway, directly activating JNK1, JNK2, and JNK3.
• Together with MAP2K7, MAP2K4 is one of only two kinases that directly phosphorylate and activate JNKs; MAP2K4 preferentially phosphorylates the tyrosine residue in the Thr-Pro-Tyr motif, while MAP2K7 targets the threonine.
• Also activates p38 MAPKs (MAPK11, MAPK12, MAPK13, MAPK14), distinguishing it from MAP2K7 which exclusively activates JNKs.
• Involved in mitochondrial death signaling, promoting cytochrome c release and apoptosis.
• Subcellular localization is primarily cytoplasmic, with some nuclear presence.
• Widely expressed with particularly abundant expression in skeletal muscle.
• Post-translational modifications include phosphorylation at S257 and other sites, acetylation, and methylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around S257; the antibody is expected to recognize phosphorylated MAP2K4 at this site. For Western blot, a band at approximately 44 kDa is anticipated.
• Include phosphatase inhibitors during sample preparation to preserve phosphorylation. Validate specificity using non-phosphorylated peptide competition or phosphatase treatment.
• For IHC-P and IF/ICC, optimize antigen retrieval and consider using phospho-specific and total protein controls to confirm localization.
• Cross-reactivity with mouse and rat allows application in rodent models; validate the antibody in the relevant tissue or cell line context.
• For ELISA and IP, titration experiments are recommended to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and research laboratories from concept to clinic. This phospho-MAP2K4 (S257) antibody complements our portfolio for studying stress-activated MAP kinase cascades, apoptosis, and immune regulation. Our extensive supply chain offers validated antibody pairs, optimized phospho-specific monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.