Anti-Phospho-LIMK2-T505 Rabbit Polyclonal Antibody for WB and IF/ICC - P53671

Core Product Specifications and Parameters

Biological Background: LIMK2 Function and Localization

• LIMK2 is a serine/threonine-protein kinase that acts downstream of Rho family GTPases, regulating actin filament dynamics and cell motility. Related references: PMID:10436159, PMID:11018042
• It phosphorylates TPPP to control astral microtubule organization and mitotic spindle orientation during early mitosis. Related references: PMID:22328514
• In vitro, LIMK2 phosphorylates myelin basic protein and histone (MBP), demonstrating broad substrate activity. Related references: PMID:8537403
• LIMK2 suppresses ciliogenesis by phosphorylating CFL1, disrupting ciliary vesicle trafficking, and driving YAP1/TEAD4-mediated transcriptional repression of AURKA and PLK1. Related references: PMID:25849865
• The protein contains LIM and PDZ domains, suggesting roles in protein-protein interactions and scaffolding.
• Localizes to the cytoplasm, cytoskeleton (spindle and centrosome), nucleus, and perinuclear region, consistent with its diverse functions.
• Classified as a transferase and phosphoprotein with metal-binding zinc finger motifs, indicative of its regulatory complexity.

Experimental Guidance and Technical Tips

• To preserve T505 phosphorylation during sample preparation, include phosphatase inhibitors in lysis buffers and avoid prolonged sample handling.
• For Western blot, expect a band at approximately 72 kDa; use fresh lysates and validate loading controls.
• In immunofluorescence, test fixation methods (e.g., methanol versus paraformaldehyde) to optimize phospho-epitope retention.
• Verify specificity using phosphatase-treated controls or peptide competition assays to confirm phospho-specific binding.
• The antibody reacts with human, mouse, and rat samples; cross-reactivity with other species should be tested empirically.
• Since the antibody is raised in rabbit, select an appropriate anti-rabbit secondary antibody for detection.

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