Anti-Phospho-KAP1/TRIM28-S824 Rabbit Monoclonal Antibody for WB, ELISA - Q13263

Core Product Specifications and Parameters

Biological Background: TRIM28 Function and Localization

• TRIM28/TIF1-beta/KAP-1 is an E3 SUMO and ubiquitin ligase that serves as a scaffold for transcriptional repression complexes, recruiting methyltransferases, histone deacetylases, and chromatin remodelers to mediate gene silencing.
• In undifferentiated embryonic stem cells, TRIM28 maintains a transcriptionally repressive state to preserve pluripotency.
  Related references: PMID:24623306
• TRIM28 is required for FOXP3-mediated repression and Treg suppressive function via ZFP90 interaction.
  Related references: PMID:23543754
• As a positive regulator of IFN-I signaling, TRIM28 promotes TBK1 ubiquitination and TBK1-IRF3 complex formation.
  Related references: PMID:38495890
• It SUMOylates TRAF6, controlling its nucleo-cytoplasmic shuttling and NF-kappa-B pathway activation.
  Related references: PMID:39920527
• TRIM28 enhances inflammasome activation by SUMOylating NLRP3, preventing its degradation.
  Related references: PMID:34373456
• Localized mainly in the nucleus, TRIM28 is ubiquitously expressed across human tissues and undergoes phosphorylation at Ser-824, which modulates its function—the target of this antibody.

Experimental Guidance and Technical Tips

• For Western blot, consider using phosphatase inhibitors in lysis and sample preparation to preserve phosphorylation at S824.
• Validate antibody specificity with lambda protein phosphatase treatment to confirm phospho-dependent signal.
• Recommended sample types include human or mouse cell lines known to express TRIM28, particularly those with activated kinase pathways targeting S824.
• For ELISA, coating with the phosphorylated peptide immunogen may serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop sourcing bridge, CamelBio offers comprehensive IVD raw-material solutions, including validated antibody pairs, optimized monoclonal/polyclonal antibodies (like phospho-specific reagents), bulk ancillary reagents, and rare target sourcing. Whether you're developing assays for epigenetic or signaling research targeting TRIM28, CamelBio can support your needs from concept to clinic.