Anti-Phospho-JunD-S255 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P17535

Core Product Specifications and Parameters

Biological Background: Transcription factor JunD Function and Localization

• Transcription factor JunD, also known as AP-1 subunit JunD, is encoded by the JUND gene.
• It functions by binding to AP-1 sites in DNA (PubMed:9989505) and forms heterodimers with FOS family proteins, enhancing their DNA binding and transcriptional activity (PubMed:28981703, PubMed:9989505).

Related references: PMID:9989505 PMID:28981703

• JunD is localized in the nucleus, consistent with its role in transcriptional regulation.
• It is a phosphoprotein, and phosphorylation at serine 255 (S255) is a key regulatory modification.
• As a member of the AP-1 transcription factor complex, JunD is involved in regulating gene expression in response to various stimuli.
• The protein has a molecular weight of approximately 35 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide corresponding to residues around S255 of human JunD; specificity for the phosphorylated form should be confirmed in appropriate controls.
• For western blotting, a band around 35 kDa is expected; consider treating samples with phosphatase to verify phosphorylation-dependent signals.
• For immunohistochemistry and ELISA applications, validate the antibody in the relevant species and sample types, as performance may vary.
• Optimal working dilutions and conditions should be determined empirically in each laboratory.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers studying AP-1 transcription factors and phosphorylated signaling pathways, this Phospho-JunD-S255 antibody is part of a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you need early-stage R&D support or scalable production, CamelBio bridges the gap between target discovery and clinical application.