Anti-Phospho-JunD (S255) Rabbit Monoclonal Antibody for WB and ELISA - P17535

Core Product Specifications and Parameters

Biological Background: Transcription factor JunD Function and Localization

• Transcription factor that binds to AP-1 consensus sites (3'-TGA[GC]TCA-5') in target gene promoters. Related references: PMID:9989505
• Heterodimerizes with FOS family proteins to form AP-1 complexes, enhancing DNA binding and transcriptional activity. Related references: PMID:28981703, PMID:9989505
• Localizes predominantly to the nucleus where it regulates gene expression.
• Functions as a transcriptional activator, regulating expression of genes involved in various cellular processes.
• As a phosphoprotein, phosphorylation at specific residues (including S255) influences JunD activity and protein interactions.
• Contains a DNA-binding domain and is involved in transcriptional regulation.

Experimental Guidance and Technical Tips

• The immunogen used was a synthetic phosphopeptide around S255 of human JunD, ensuring specificity for the phosphorylated form. For detection of total JunD, a pan-specific antibody is recommended.
• For Western blot (WB), use phosphatase inhibitors during cell lysis to maintain phosphorylation. Expect a band at approximately 35 kDa in human and mouse samples. Validate with phosphatase-treated controls.
• For ELISA, optimize coating and detection conditions for phosphorylated JunD detection in cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For AP-1 transcription factor research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities also extend to rare target raw materials, ensuring you have the critical reagents needed for your diagnostic development.