Anti-Phospho-JAK2 (Y1007/1008) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O60674

Core Product Specifications and Parameters

Biological Background: JAK2 Function and Localization

• Tyrosine-protein kinase JAK2 (Janus kinase 2) is a non-receptor protein tyrosine kinase that plays a central role in cytokine receptor signaling.
• It phosphorylates type I receptors (such as GHR, PRLR, EPOR) and type II receptors (such as IFN receptors), creating phosphotyrosine-docking sites for downstream signaling molecules (PMID:15690087, PMID:15899890, PMID:7615558). Related references: PMID:15690087 PMID:15899890 PMID:7615558
• After ligand binding, JAK2 also phosphorylates STAT proteins (e.g., STAT5, STAT3) once they are recruited to the receptor, leading to their dimerization and nuclear translocation to activate gene transcription (PMID:15690087, PMID:9618263). Related references: PMID:15690087 PMID:9618263
• In erythropoiesis, erythropoietin (EPO) stimulation triggers JAK2 autophosphorylation, association with the EPOR, and subsequent STAT5 recruitment and phosphorylation, driving transcription of genes essential for red blood cell development (PMID:9657743). Related references: PMID:9657743
• JAK2 mediates chromatin regulation by directly phosphorylating histone H3 at tyrosine 41 (H3Y41ph), a modification that excludes the heterochromatin protein HP1α (CBX5) from chromatin, thereby modulating gene expression (PMID:19783980). Related references: PMID:19783980
• JAK2 is ubiquitously expressed in most tissues, supporting its pleiotropic roles in growth, development, and immunity.
• Subcellularly, JAK2 localizes to the cytoplasm, nucleus, and endomembrane system, enabling both membrane-proximal signaling and direct nuclear actions.
• It is classified as a tyrosine-protein kinase, phosphoprotein, and proto-oncogene, with a SH2 domain, and is implicated in innate and adaptive immunity, chromosomal rearrangements, and disease.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic phosphopeptide encompassing the Y1007 and Y1008 residues of human JAK2, which are critical for kinase activation. This antibody is intended to detect only the phosphorylated (active) form of JAK2.
• In Western blotting, a specific band at ~131 kDa is expected. To confirm phospho-specificity, consider treating samples with lambda phosphatase or using JAK2 kinase inhibitors as controls.
• For IHC-P, optimal results may require antigen retrieval and titration of primary antibody; include positive and negative control tissues known to have active JAK2 signaling.
• In ELISA, this antibody can serve as a detection component when paired with a capture antibody recognizing total JAK2 or a distinct epitope.
• Given the broad roles of JAK2 in cytokine signaling, consider validating the antibody in your specific experimental system, as activation status can vary with cell type and treatment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides research and diagnostic manufacturers with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-Phospho-JAK2 (Y1007/1008) antibody is tailored for investigations into cytokine signaling, hematopoiesis, and oncology. For those requiring rare target reagents or scalable sourcing solutions, CamelBio’s integrated supply pipeline and technical expertise help accelerate your developmental and commercial milestones.