Anti-Phospho-JAK2-Y1007 Rabbit Polyclonal Antibody - O60674

Core Product Specifications and Parameters

Biological Background: JAK2 Function and Localization

• JAK2 (Janus kinase 2) is a non-membrane spanning tyrosine kinase that initiates signaling from multiple type I and type II cytokine receptors, including those for growth hormone, prolactin, leptin, erythropoietin, thrombopoietin, interferons, and interleukins. Related references: PMID:15690087, PMID:15899890, PMID:7615558

• Following ligand binding, JAK2 phosphorylates receptor cytoplasmic tails, creating docking sites for STATs, then phosphorylates STATs (e.g., STAT5, STAT3), which dimerize and translocate to the nucleus to regulate gene expression. Related references: PMID:15690087, PMID:9618263

• In erythropoiesis, erythropoietin (EPO) stimulation leads to JAK2 autophosphorylation and activation, recruitment and phosphorylation of STAT5, and transcriptional induction of erythroid-specific genes. Related references: PMID:9657743

• In the nucleus, JAK2 directly phosphorylates histone H3 at Tyr41 (H3Y41ph), a modification that evicts HP1α from chromatin and alters gene expression, linking cytokine signaling to epigenetic regulation. Related references: PMID:19783980

• JAK2 also participates in angiotensin-2-induced ARHGEF1 phosphorylation, cell cycle control via CDKN1B phosphorylation, and regulation of potassium channel KCNA3 activity. Related references: PMID:20098430, PMID:21423214, PMID:25644777

• JAK2 is ubiquitously expressed in human tissues and localizes to the cytoplasm, nucleus, and endomembrane system, consistent with its roles in both cytoplasmic signaling and nuclear chromatin modulation.

• Cooperates with TEC kinase to drive FOS transcription and is implicated in innate and adaptive immunity, as well as being classified as a proto-oncogene due to its involvement in myeloproliferative disorders.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphopeptide around Tyr1007 of human JAK2; it is designed to specifically detect JAK2 when phosphorylated at this residue.
• For Western blotting, a major band at ~131 kDa is expected. Include phosphatase inhibitors in the lysis buffer to preserve phosphorylation.
• The antibody has been validated for use in WB and ELISA applications; optimization of dilution and conditions is recommended for each experimental setup.
• Known cross-reactivity includes human and mouse JAK2; reactivity with other species or related kinases (e.g., JAK1, JAK3, TYK2) should be verified independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing of IVD raw materials, including validated phospho-specific antibodies like this anti-pY1007 JAK2 antibody, supporting research in cytokine signaling, hematology, and oncology. As your one-stop partner from concept to clinic, we also supply optimized antibody pairs, bulk ancillary reagents, and rare target raw materials, enabling seamless development of diagnostic immunoassays and laboratory tests.