Anti-Phospho-JAK2 (Y1007) Rabbit Polyclonal Antibody for WB and ELISA - O60674

Core Product Specifications and Parameters

Biological Background: JAK2 Function and Localization

• JAK2 is a non-receptor tyrosine-protein kinase that serves as a critical mediator of signal transduction for a wide range of cytokine and growth factor receptors, including GHR, PRLR, LEPR, EPOR, TPOR, and interferon receptors. Upon ligand binding, JAK2 phosphorylates specific receptor tyrosine residues, creating docking sites for STAT proteins, and subsequently phosphorylates STATs, leading to their dimerization, nuclear translocation, and target gene activation. Related references: PMID:15690087 PMID:9618263 PMID:7615558
• JAK2 plays a central role in erythropoiesis by mediating EPO signaling. EPO stimulation triggers JAK2 autophosphorylation, activation, and phosphorylation of EPOR, followed by recruitment and phosphorylation of STAT5, ultimately promoting transcription of genes essential for red blood cell production. Related references: PMID:9657743
• In the nucleus, JAK2 functions as a chromatin regulator by phosphorylating tyrosine 41 of histone H3 (H3Y41ph), a modification that excludes the heterochromatin protein HP1α (CBX5) from chromatin, thereby modulating gene expression. Related references: PMID:19783980
• JAK2 is involved in multiple other signaling cascades, including cellular retinol-induced STAT5 activation, angiotensin-2-mediated ARHGEF1 phosphorylation, cell cycle regulation via CDKN1B phosphorylation, and reciprocal activation with TEC to induce FOS transcription. Related references: PMID:21368206 PMID:20098430 PMID:21423214
• The protein is ubiquitously expressed across most tissues and localizes to the cytoplasm and nucleus, as well as the endomembrane system, consistent with its versatile signaling roles.
• JAK2 contains an SH2 domain, kinase domain, and pseudokinase domain, and undergoes regulatory phosphorylation and ubiquitination. It is classified as a proto-oncogene, with chromosomal rearrangements implicated in diseases such as myeloproliferative neoplasms.
• This antibody specifically detects JAK2 phosphorylated at tyrosine 1007, a key autophosphorylation site essential for full kinase activation and downstream signaling.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide surrounding residue Y1007 of human JAK2, ensuring high specificity for the phosphorylated epitope. For optimal results, consider validating the antibody in relevant positive control lysates, such as cytokine-stimulated cells (e.g., EPO-treated erythroid progenitors or IL-3–stimulated hematopoietic cells).
• For Western blot, use phosphatase inhibitors in lysis buffers to preserve phosphorylation signals, and evaluate the antibody at concentrations based on preliminary titration experiments in your sample system. The predicted molecular weight is 131 kDa.
• This antibody is recommended for WB and ELISA applications; performance in other applications should be tested by the end user.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio connects diagnostic developers and research labs with a comprehensive IVD raw‑material portfolio, including phospho‑specific antibodies like this anti‑JAK2(pY1007) antibody for JAK/STAT pathway studies. Our offerings span validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare‑target sourcing—all designed to support your assay development from concept to clinic. Let us simplify your procurement with reliable, one‑stop access to critical reagents.