Anti-Phospho-IRS1-S636 Rabbit Polyclonal Antibody for WB - P35568

Core Product Specifications and Parameters

Biological Background: Insulin Receptor Substrate 1 (IRS1) Function and Localization

• IRS1, also known as HIRS-1, is a critical signaling adapter for the insulin receptor (INSR) and IGF1R, mediating metabolic and mitogenic responses. Related references: PMID:7541045 PMID:33991522 PMID:38625937

• Upon tyrosine phosphorylation, IRS1 recruits SH2 domain proteins (PI3K, GRB2, NCK1/2, SHP2), activating the PI3K/AKT and Ras/MAPK pathways to regulate metabolism, growth, and gene expression. Related references: PMID:11171109 PMID:8265614

• IRS1 plays an essential role in development, growth, glucose homeostasis, and lipid metabolism. Related references: PMID:19639489

• IRS1 positively regulates Wnt/β-catenin signaling by preventing autophagy-driven degradation of DVL2, enhancing cell proliferation. Related references: PMID:24616100

• The protein localizes to both cytoplasm and nucleus, enabling proximal signal transduction and potential transcriptional functions.

• IRS1 is extensively phosphorylated; serine phosphorylation at S636 can modulate insulin signaling, and dysregulation is associated with diabetes mellitus.

Experimental Guidance and Technical Tips

• This phospho-specific antibody was raised against a peptide surrounding S636; ensure that samples are prepared with phosphatase inhibitors to preserve phosphorylation status.
• For Western blot validation, consider using positive controls such as insulin-stimulated cell lysates and compare with untreated or phosphatase-treated samples.
• The predicted molecular weight is ~132 kDa; confirm band specificity by comparing to total IRS1 antibody signals.
• As cross-reactivity is reported for human and mouse, validate in the relevant species’ tissue or cell lines.
• Optimize antibody dilution and exposure conditions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD manufacturers and researchers, offering a comprehensive portfolio of validated antibodies, antigen pairs, and ancillary reagents. This IRS1 phospho-specific antibody supports studies in insulin signaling, metabolic disorders, and oncology. Beyond catalog products, we provide tailored sourcing for rare targets and technical consultation to accelerate your diagnostic development from concept to clinic.