Anti-Phospho-IRS1-S612 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P35568

Core Product Specifications and Parameters

Biological Background: IRS1 Function and Localization

• Insulin receptor substrate 1 (IRS1) is a signaling adapter protein that mediates signal transduction from the insulin receptor (INSR) and insulin-like growth factor I receptor (IGF1R), playing a critical role in development, growth, glucose homeostasis, and lipid metabolism. Related references: PMID:7541045, PMID:33991522, PMID:38625937, PMID:19639489
• Upon phosphorylation by the insulin receptor, IRS1 functions as a signaling scaffold that propagates insulin action by binding to SH2 domain-containing proteins, including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2, and SHP2. Related references: PMID:11171109, PMID:8265614
• Recruitment of GRB2 activates the Ras/Raf/MEK/MAPK pathway, while PI3K recruitment triggers the PI3K/AKT pathway, coordinating metabolic and mitogenic responses.
• IRS1 acts as a positive regulator of the Wnt/beta-catenin signaling pathway by suppressing DVL2 autophagy-mediated degradation, thereby promoting cell proliferation. Related references: PMID:24616100
• Subcellular localization includes the cytoplasm and nucleus.
• IRS1 undergoes extensive post-translational modifications, including phosphorylation, S-nitrosylation, and ubiquitination. This antibody specifically detects phosphorylation at serine 612, a regulatory site in IRS1 signaling.
• Genetic variations in IRS1 are associated with susceptibility to diabetes mellitus.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S612; therefore, the antibody is expected to specifically recognize IRS1 phosphorylated at this site. For western blot, consider confirming phospho-specificity through lambda phosphatase treatment or phospho-peptide competition assays.
• The predicted molecular weight of IRS1 is approximately 132 kDa. When performing WB, use an appropriate gel percentage (e.g., 8% SDS-PAGE) and optimize transfer conditions for high molecular weight proteins.
• Cross-reactivity with mouse and rat suggests conservation of the epitope sequence; however, validation in the relevant sample system is recommended.
• For IHC-P, antigen retrieval may be required, and the addition of phosphatase inhibitors during tissue processing can help preserve phosphorylation. Primary antibody dilution and incubation should be optimized empirically.
• In ELISA applications, coating with the phosphorylated peptide is recommended, and detection can be performed using standard indirect ELISA protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a reliable one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutes from concept to clinic. This phospho-specific IRS1 antibody is particularly suited for research into insulin signaling, metabolic disorders, and diabetes. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to accelerate your assay development.