Anti-Phospho-IRF3 (Ser386) Rabbit Monoclonal Antibody for WB, ELISA - Q14653

Core Product Specifications and Parameters

Biological Background: IRF3 Function and Localization

• IRF3 is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses, essential for innate immune defense against DNA and RNA viruses. Related references: PMID:22394562 PMID:24049179 PMID:25636800
• It binds to interferon-stimulated response elements (ISRE) in the promoters of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISGs) to activate their transcription. Related references: PMID:11846977 PMID:16846591 PMID:16979567
• Acts as a more potent activator of IFN-beta than IFN-alpha and is critical in both early and late phases of the IFNA/B gene induction. Related references: PMID:16846591 PMID:16979567 PMID:20049431
• In uninfected cells, IRF3 resides in the cytoplasm in an inactive form. Upon viral infection, dsRNA, or TLR signaling, it is phosphorylated by IKBKE and TBK1 kinases, which induces conformational change, dimerization, and nuclear translocation. In the nucleus, it associates with CREBBP to form the DRAF1 complex, activating target gene transcription. Related references: PMID:22394562 PMID:25636800 PMID:27302953 PMID:16154084 PMID:33440148
• IRF3 can drive distinct gene expression programs in macrophages and induce apoptosis in primary macrophages. Related references: PMID:16846591
• In response to Sendai virus infection, IRF3 is recruited to mitochondrion by TOMM70:HSP90AA1, forming an apoptosis complex with BAX. Related references: PMID:25609812
• It also functions as a key transcription factor during SARS-CoV-2 infection, regulating the IFN response. Related references: PMID:33440148

Experimental Guidance and Technical Tips

• Since this antibody recognizes phospho-Ser386, include phosphatase inhibitors in lysis buffers and consider phosphatase treatment of samples as a negative control to confirm specificity.
• For Western blotting, the predicted molecular weight of IRF3 is 47 kDa; phospho-specific bands may be faint due to low basal phosphorylation, so optimize exposure times.
• In ELISA applications, ensure the coating antigen is phosphorylated at Ser386 to maintain epitope recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, researchers, and laboratories with a comprehensive portfolio of IVD raw materials, including phospho-specific antibodies like this anti-phospho-IRF3 (Ser386) monoclonal antibody. Our one-stop sourcing platform covers validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to support your assay development from concept to clinic. For customized solutions in innate immunity and viral infection research, contact our team.