Anti-Phospho-IRAK4 (Thr345/Ser346) Rabbit Polyclonal Antibody for WB and ELISA - Q9NWZ3

Core Product Specifications and Parameters

Biological Background: IRAK4 Function and Localization

• IRAK4 (Interleukin-1 receptor-associated kinase 4), also known as renal carcinoma antigen NY-REN-64, is a serine/threonine-protein kinase critical for innate immune responses.
• It functions in Toll-like receptor (TLR) and IL-1 receptor (IL-1R) signaling pathways, rapidly recruited by MYD88 to form the Myddosome complex upon receptor activation. Related references: PMID:17878374
• IRAK4 phosphorylates IRAK1, stimulating its kinase activity and autophosphorylation, and also phosphorylates Pellino E3 ubiquitin ligases (PELI1-3) to promote polyubiquitination of IRAK1, leading to NF-kB activation.
• Alternatively, IRAK4 phosphorylates TIRAP to promote its ubiquitination and degradation, modulating signaling.
• IRAK4 is localized in the cytoplasm and is a phosphoprotein; phosphorylation at residues Thr345 and Ser346 (targeted by this antibody) is important for its kinase function.
• Key post-translational modifications include phosphorylation and acetylation; the protein is also subject to alternative splicing.
• Disease-associated variants of IRAK4 are linked to immunodeficiencies, underscoring its role in host defense.

Experimental Guidance and Technical Tips

• For Western blotting, use a phospho-specific protocol with appropriate blocking buffers and consider phosphatase inhibitor cocktails during sample preparation to preserve phosphorylation.
• Validate antibody specificity by treating lysates with lambda phosphatase; disappearance of the band confirms phospho-specificity.
• For ELISA, coat plates with the phosphopeptide immunogen or whole cell lysates from stimulated cells, and optimize concentrations.
• This antibody is validated for human samples; cross-reactivity with other species should be tested independently.
• Since the antibody is rabbit polyclonal, use an anti-rabbit HRP-conjugated secondary antibody for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. This anti-phospho-IRAK4 (Thr345/Ser346) antibody supports research into innate immunity, TLR signaling, and related disorders. From early-stage R&D to clinical supply, we provide reliable access to critical reagents like this phospho-specific antibody, accelerating your assay and kit development.