Anti-Phospho-INSR-Y1150/IGF1R-Y1135 Rabbit Polyclonal Antibody for WB, ELISA - P06213 / P08069

Core Product Specifications and Parameters

Biological Background: INSR and IGF1R Function and Localization

• INSR is a receptor tyrosine kinase that mediates insulin's pleiotropic metabolic and growth-promoting effects through activation of PI3K-AKT and Ras-MAPK signaling cascades.
• Insulin-induced phosphorylation of IRS proteins leads to PI3K-AKT pathway activation, promoting glucose transporter translocation, anti-apoptosis, and regulation of gluconeogenic/lipogenic enzymes.
• The Ras-MAPK pathway, triggered via GRB2/SOS recruitment to phosphorylated IRS1, controls cell growth, survival, and differentiation.
• INSR also binds IGF1 and IGF2 and can form hybrid receptors with IGF1R; these hybrid receptors exhibit distinct ligand affinities depending on isoform composition.
  Related references: PMID:12138094 PMID:16831875
• INSR is predominantly expressed in insulin target tissues (liver, adipose, skeletal muscle) and is overexpressed in breast, colon, lung, ovary, and thyroid carcinomas.
• Subcellular localization encompasses the cell membrane, late endosomes, and lysosomes, with receptor trafficking modulating signal duration and specificity.
• The antibody specifically detects phosphorylation at Y1150 (INSR) and Y1135 (IGF1R) within the kinase activation loop, a modification essential for full receptor kinase activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y1150 of human INSR/IGF1R; this design targets the activation loop phosphorylation site specifically.
• For Western blotting, consider using whole‑cell lysates from insulin‑stimulated human, mouse, or rat cell lines; expect a band near 154–156 kDa.
• In ELISA applications, use a phosphorylated peptide as coating antigen and include a non‑phosphopeptide control to verify phospho‑specificity.
• Include phosphatase inhibitors during sample preparation and optimize cell stimulation conditions to preserve phosphorylation signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one‑stop sourcing partner for IVD raw materials, serving diagnostic manufacturers, labs, and research institutes from concept to clinic. This phospho‑INSR/IGF1R antibody supports studies in insulin signaling, metabolic disease, and oncology. We offer validated antibody pairs, bulk ancillary reagents, and procurement of rare‑target raw materials to accelerate your diagnostic development.