Anti-Phospho-IKKα-S180 Rabbit Polyclonal Antibody for WB, ELISA - O15111

Core Product Specifications and Parameters

Biological Background: IKKα Function and Localization

• IKKα (CHUK) is a serine/threonine kinase that acts as the catalytic subunit of the IKK complex in the canonical NF-kappa-B pathway, phosphorylating IκB inhibitors to trigger their degradation and NF-kappa-B activation (PubMed:18626576, PubMed:9244310, PubMed:9252186).

• In the non-canonical NF-kappa-B pathway, IKKα homodimers phosphorylate NFKB2/p100, leading to its processing to p52 and formation of RelB-p52 complexes essential for B-cell survival and lymphoid organogenesis (PubMed:20501937).

• IKKα exerts negative feedback on NF-kappa-B signaling by phosphorylating TAX1BP1, facilitating the assembly of the A20 ubiquitin-editing complex that limits inflammatory gene activation (PubMed:21765415).

• Within the nucleus, IKKα phosphorylates histone H3 at Ser10, promoting subsequent acetylation by CREBBP and modulating chromatin accessibility at NF-kappa-B-responsive promoters (PubMed:12789342). It also phosphorylates CREBBP itself to enhance its transcriptional and histone acetyltransferase activities (PubMed:17434128).

• IKKα is widely expressed and localizes to both the cytoplasm and nucleus, consistent with its dual roles in signal transduction and nuclear gene regulation.

• Post-translational modifications include phosphorylation (key for kinase activation) and acetylation, and the protein is a known phosphoprotein.

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic phosphopeptide around the Ser180 phosphorylation site of human IKKα. It is designed to recognize the phosphorylated form; confirm specificity using phosphatase treatment or non-phosphopeptide competition in your assay.

• For Western blot, consider loading appropriate positive controls (e.g., cells stimulated with TNF-α or IL-1β) alongside untreated samples, and use phosphatase inhibitors during lysis to preserve phosphorylation.

• The recommended applications are Western blot and ELISA based on supplier specifications; optimal dilutions should be determined experimentally in your sample system.

• Cross-reactivity is indicated for human and mouse; for other species, validate based on sequence homology of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories in procuring high-quality IVD raw materials, including this phospho-specific IKKα antibody for NF-kappa-B signaling studies. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and a wide range of ancillary reagents. For specialized needs in kinase biology or inflammatory disease research, our team can facilitate customized sourcing to streamline your development from concept to clinical application.