Anti-Phospho-IκBα (Ser36) Rabbit Polyclonal Antibody for WB and ELISA - P25963

Core Product Specifications and Parameters

Biological Background: IκBα (NF-κB Inhibitor Alpha) Function and Localization

• IκBα (NF-κB inhibitor alpha), encoded by the NFKBIA gene, is a critical regulator of the NF-κB signaling pathway, functioning primarily to retain NF-κB dimers in the cytoplasm.
  Related references:
  PMID:1493333, PMID:36651806, PMID:7479976

• Upon stimulation by cytokines or pathogens, IκBα is phosphorylated at serine residues 32 and 36, which targets it for polyubiquitination and proteasomal degradation, allowing NF-κB nuclear translocation and gene activation.
  Related references:
  PMID:7479976, PMID:7628694, PMID:7796813

• The protein contains multiple ankyrin repeats that mediate its interaction with the p65 and p50 subunits of NF-κB, masking their nuclear localization signals.

• IκBα shuttles between the cytoplasm and nucleus; it can enter the nucleus to remove NF-κB from DNA and re-sequester it in the cytoplasm, ensuring transient transcriptional responses.

• Post-translational modifications, including phosphorylation, ubiquitination, hydroxylation, and isopeptide bond formation, tightly regulate IκBα stability and function.

• Mutations in NFKBIA are associated with ectodermal dysplasia with immunodeficiency (EDA-ID), underscoring its essential role in immune system development and homeostasis.

• Viral pathogens often target IκBα to modulate NF-κB signaling, highlighting its involvement in host-virus interactions.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphopeptide corresponding to Ser36 of human IκBα; it likely specifically detects IκBα only when phosphorylated at this residue. Validation of phospho-specificity using phosphatase treatment or non-phosphorylated peptide competition is advised.
• For Western blot, stimulate cells (e.g., with TNF-α or IL-1β) to induce robust IκBα phosphorylation. A time-course experiment may be required to capture the transient nature of this modification.
• Based on sequence similarity, the antibody is expected to cross-react with mouse and rat phospho-IκBα. However, verifying the peptide sequence alignment around Ser36 in these species is recommended.
• For ELISA applications, the antibody may be used as a capture or detection reagent in a sandwich ELISA format. Optimize coating and detection conditions empirically.
• Avoid repeated freeze-thaw cycles; store antibody at -20°C upon receipt to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive provider of IVD raw materials, bridging the gap between research and clinical application with a diverse portfolio of antibodies, antigens, and ancillary reagents. For researchers and manufacturers focusing on NF-κB signaling, we offer this phospho-specific anti-IκBα (Ser36) polyclonal antibody along with matched detection reagents and custom sourcing options for rare targets. Our integrated technical support and procurement solutions streamline assay development from concept to clinic, ensuring reliable supply and consistent performance for diagnostic and research workflows.