Anti-Phospho-IκBα (Ser32) Rabbit Monoclonal Antibody for WB, ELISA - P25963

Core Product Specifications and Parameters

Biological Background: IκBα Function and Localization

• NF-κB inhibitor alpha (IκBα) is a key regulator of NF-κB transcription factors, encoded by the NFKBIA gene. It is also known as I-kappa-B-alpha, IkB-alpha, and MAD3.
• IκBα functions by binding to NF-κB dimers (such as RELA/p65 and NFKB1/p50) in the cytoplasm, masking their nuclear localization signals and preventing nuclear translocation. Related references: PMID:1493333, PMID:36651806, PMID:7479976
• Upon pro-inflammatory or immune stimulation, IκBα undergoes phosphorylation at serine residues 32 and 36, leading to ubiquitination and proteasomal degradation, releasing NF-κB to translocate to the nucleus and activate transcription. Related references: PMID:7479976, PMID:7628694, PMID:7796813
• IκBα predominantly localizes to the cytoplasm but can shuttle into the nucleus, where it may also inhibit NF-κB DNA binding or mediate termination of NF-κB response.
• Phosphorylation at Ser32 is a critical event in the canonical NF-κB signaling pathway, making it a widely studied biomarker for pathway activation.
• Keywords from UniProt indicate that IκBα is a phosphoprotein, undergoes ubiquitination, and is involved in host-virus interactions, highlighting its central role in immune signaling and disease.
• The protein has a molecular weight of approximately 35.6 kDa (calculated) and contains ankyrin repeat domains that mediate interactions with NF-κB.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser32 of human IκBα; therefore, the antibody specifically recognizes the phosphorylated form. Always include phosphatase inhibitors during sample preparation for Western blot to preserve the phosphorylation signal.
• For ELISA applications, the antibody can be used as a capture or detection reagent for quantifying phospho-IκBα in cell or tissue lysates; consider pairing with a total IκBα antibody for normalization.
• Confirm antibody specificity in your system by treating samples with lambda phosphatase or by using a non-phosphorylated peptide as a competitor.
• Cross-reactivity is reported for human, mouse, and rat. Validate reactivities in the relevant species before large-scale experiments, as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides streamlined access to high-quality IVD raw materials like the anti-phospho-IκBα (Ser32) rabbit monoclonal antibody, essential for NF-κB pathway research and diagnostic assay development. We offer validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions—bridging your project from concept to clinic. Whether you need rare target raw materials or optimized monoclonal/polyclonal antibodies, our B2B support ensures reliable supply for your manufacturing and research needs.