Anti-Phospho-IGF-I Receptor β (Tyr1135/1136) / Insulin Receptor β (Tyr1150/1151) Rabbit Polyclonal Antibody for WB and ELISA - P06213/P08069

Core Product Specifications and Parameters

Biological Background: Insulin Receptor (INSR) / IGF-I Receptor (IGF1R) Function and Localization

• Insulin receptor (INSR) is a receptor tyrosine kinase that mediates the pleiotropic actions of insulin, including metabolic regulation, cell growth, and differentiation.
• Upon insulin binding, INSR phosphorylates intracellular substrates such as IRS1, SHC, and CBL, leading to activation of two major signaling pathways: the PI3K-AKT/PKB pathway (responsible for metabolic actions) and the Ras-MAPK pathway (regulating cell growth and differentiation).
• INSR can form hybrid receptors with IGF1R; these hybrids bind IGF1 with high affinity, while their insulin sensitivity varies depending on the isoform composition. Related references: PMID:12138094 PMID:16831875
• INSR localizes to the cell membrane and, following activation, can internalize into late endosomes and lysosomes for signaling attenuation or degradation.
• Two main isoforms (Long and Short) are predominantly expressed in insulin target tissues (liver, adipose, skeletal muscle), with Short also expressed in fetal cells; INSR is overexpressed in several tumors including breast, colon, and lung carcinomas.
• Key post-translational modifications include tyrosine phosphorylation essential for kinase activity, as well as glycosylation, disulfide bond formation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to the activation loop phosphorylation sites; consider verifying phospho-specificity by treating samples with λ-phosphatase prior to Western blotting.
• For Western blot, insulin-stimulated cell lysates (e.g., HepG2, 3T3-L1 adipocytes) are recommended to induce phosphorylation at the target sites; a band around 156 kDa is expected for the β-subunit.
• In ELISA, the antibody may be used to capture or detect phospho-receptors; optimization of coating and detection conditions is advised.
• The reported reactivity is against human; cross-reactivity with other species is possible but should be validated experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-phospho-IGF1R/INSR antibody facilitates insulin and IGF signaling pathway investigations, and CamelBio offers a full portfolio of validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Contact us to streamline your assay development with reliable, quality-controlled reagents.