Anti-Phospho-HSP27/HSPB1-S78 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P04792

Core Product Specifications and Parameters

Biological Background: HSP27/HSPB1 Function and Localization

• HSPB1 is a small heat shock protein functioning as an ATP-independent molecular chaperone that maintains denatured proteins in a folding-competent state under stress conditions. Related references: PMID:10383393 PMID:20178975
• It plays critical roles in stress resistance and actin cytoskeleton organization. Related references: PMID:19166925
• Chaperone activity of HSPB1 regulates phosphorylation and axonal transport of neurofilament proteins, implicating it in neuronal maintenance. Related references: PMID:23728742
• The protein is expressed in all tissues tested, with highest levels in heart and striated/smooth muscle, consistent with stress-responsive roles.
• Subcellular localization includes cytoplasm, nucleus, and association with the spindle, reflecting its dynamic distribution.
• HSPB1 undergoes multiple post-translational modifications including phosphorylation at Ser78, acetylation, and methylation, modulating its chaperone function.
• Mutations in HSPB1 are linked to Charcot-Marie-Tooth disease and other neuropathies.
• Keywords: Chaperone, Stress response, Phosphoprotein, Cytoplasm, Nucleus, Cytoskeleton, Disease variant.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phospho-peptide around Ser78; specificity for the phosphorylated form should be confirmed using phosphatase-treated lysates or non-phospho peptide competition.
• For Western blot, expect a band at ~23 kDa (full-length HSPB1). Use standard reducing conditions and consider loading controls appropriate for the tissue or cell type.
• In immunofluorescence/ICC, optimize fixation and permeabilization protocols, as HSPB1 can localize to both cytoplasmic and nuclear compartments.
• This antibody is validated on human samples; cross-reactivity with other species is not guaranteed and should be verified empirically if needed.
• For ELISA applications, pairing with a suitable capture antibody or antigen may be required; titrate the antibody to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a reliable one-stop sourcing channel for IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare-target antibodies. Focusing on the HSP27/HSPB1 stress-response chaperone, our supply scope covers phospho-specific and total antibodies suitable for assay development. For projects requiring customized or hard-to-find raw materials, our technical team helps bridge the gap from concept to clinic.