Anti-Phospho-HSP27/HSPB1-S78 Rabbit Monoclonal Antibody for WB and ELISA - P04792

Core Product Specifications and Parameters

Biological Background: HSPB1 (Heat Shock Protein Beta-1) Function and Localization

• HSPB1 (also known as HSP27) is a small heat shock protein that functions as a molecular chaperone, maintaining denatured proteins in a folding-competent state. Related references: PMID:10383393 PMID:20178975
• It plays a role in stress resistance and actin organization. Related references: PMID:19166925
• Through its chaperone activity, HSPB1 may regulate the phosphorylation and axonal transport of neurofilament proteins. Related references: PMID:23728742
• Subcellular localization includes cytoplasm, nucleus, cytoskeleton, and mitotic spindle.
• HSPB1 is broadly detected in human tissues, with highest expression in heart and striated/smooth muscle tissues.
• The protein undergoes multiple post-translational modifications including phosphorylation, acetylation, and methylation, which modulate its chaperone function and interactions.
• Mutations in HSPB1 are associated with Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (HMN2B).

Experimental Guidance and Technical Tips

• This antibody is generated against a phosphorylated peptide around serine 78; it is expected to preferentially recognize the phosphorylated form of HSPB1. Verify specificity using phosphatase-treated samples or competition with non-phosphorylated peptide.
• For western blot, a specific band near 23 kDa should be observed. Use appropriate positive controls (e.g., heat-shocked cells) and consider loading controls to confirm detection.
• ELISA performance may require optimization of coating antigen and detection conditions; cross-reactivity with non-phosphorylated HSPB1 should be ruled out by parallel assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and IVD developers with one-stop access to high-quality raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-Phospho-HSPB1-S78 antibody is part of our portfolio for stress response and chaperone research, supporting assay development in areas such as oncology, neurodegeneration, and inflammation. As a procurement partner, we streamline supply chains from concept to clinic, ensuring consistent quality and availability for your diagnostic and research needs.