Anti-Phospho-HSP27/HSPB1 (S15) Polyclonal Antibody for WB, IP, ELISA - P04792

Core Product Specifications and Parameters

Biological Background: HSP27/HSPB1 Function and Localization

• HSP27/HSPB1 is a small heat shock protein (also known as 28 kDa heat shock protein, HSP27, HSP28, SRP27) that belongs to the HSPB family. It functions as a molecular chaperone, maintaining denatured proteins in a folding-competent state.
  Related references: PMID:10383393 PMID:20178975
• HSP27/HSPB1 plays a critical role in stress resistance and actin cytoskeleton organization.
  Related references: PMID:19166925
• Through its chaperone activity, HSP27/HSPB1 regulates phosphorylation and axonal transport of neurofilament proteins, important for neuronal function.
  Related references: PMID:23728742
• Subcellular localization: predominantly cytoplasmic, but also found in the nucleus, associated with the cytoskeleton and mitotic spindle.
• Tissue distribution: ubiquitously expressed in all tissues tested, with highest levels in heart and striated/smooth muscle tissues (skeletal muscle, aorta, etc.).
• HSP27/HSPB1 is a phosphoprotein with multiple phosphorylation sites (including S15); it is also acetylated and methylated. Mutations in HSPB1 are associated with Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy.

Experimental Guidance and Technical Tips

• Because this antibody targets a phosphorylation-specific site (S15), confirm specificity by performing phospho-peptide competition assays or treating samples with phosphatase before detection.
• For western blot analysis, include phosphatase inhibitors in the lysis buffer to preserve phosphorylation signals.
• Validate the antibody in relevant human cell lines or tissue samples that express HSP27/HSPB1, especially under stress conditions known to induce phosphorylation.
• Consider using positive and negative controls (e.g., heat-shocked vs. untreated cells) to verify phospho-specific detection in IP or ELISA experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined sourcing experience for IVD raw materials. This anti-phospho-HSP27/HSPB1 (S15) antibody supports research into stress response, chaperone biology, and neurodegenerative disorders. Alongside specialized antibodies like this, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your development pipeline.