Anti-Phospho-HSL (Ser660) Rabbit Polyclonal Antibody for WB - Q05469

Core Product Specifications and Parameters

Biological Background: Hormone-Sensitive Lipase Function and Localization

• Hormone-sensitive lipase (HSL), encoded by the LIPE gene, is a multifunctional lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols, diacylglycerols, monoacylglycerols, cholesteryl esters, and retinyl esters. Related references: PMID:15716583, PMID:15955102, PMID:19800417
• It preferentially hydrolyzes diacylglycerols over triacylglycerols and monoacylglycerols, with a preference for fatty acid esters at the sn-3 position in DAGs.
• HSL also hydrolyzes 2-arachidonoylglycerol (an endocannabinoid) and 2-acetyl monoalkylglycerol ether (a precursor for platelet-activating factor) by similarity.
• In adipose tissue and heart, HSL primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it converts cholesteryl esters to free cholesterol for steroid hormone production.
• Alternative splicing generates tissue-specific isoforms; the protein is highly expressed in testis.
• Subcellular localization includes the cell membrane, caveolae, cytoplasm, cytosol, and lipid droplets, reflecting its dynamic role in lipid mobilization.
• Post-translational modifications include phosphorylation at Ser660, which is critical for enzyme activation and is a key regulatory event in lipolysis.
• Keywords associated with HSL include cholesterol metabolism, lipid degradation, diabetes mellitus, and obesity, highlighting its significance in metabolic disease research.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide corresponding to human HSL phosphorylated at Ser660; verify specificity by treating cell lysates with phosphatase to confirm loss of signal.
• For Western blot, a band at approximately 117 kDa is expected; however, multiple isoforms and phosphorylation states may result in additional bands.
• Consider including appropriate positive and negative controls, such as stimulated vs. unstimulated adipocyte lysates, to confirm phospho-specific detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, including validated phospho-specific antibodies like this anti-Phospho-HSL (Ser660) antibody for lipid metabolism research. Our comprehensive portfolio covers optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, ensuring seamless integration from concept to clinic.