Anti-Phospho-HSL-S660 Rabbit Polyclonal Antibody - Q05469

Core Product Specifications and Parameters

Biological Background: Hormone-sensitive lipase Function and Localization

• Hormone-sensitive lipase (HSL), encoded by the LIPE gene, is a multifunctional enzyme with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters, and retinyl esters.
  Related references:
  PMID:15716583, PMID:15955102, PMID:19800417
• It exhibits a preference for DAGs over TAGs and MAGs, and preferentially hydrolyzes fatty acid esters at the sn-3 position of the glycerol backbone in DAGs.
  Related references:
  PMID:19800417
• HSL also hydrolyzes 2-arachidonoylglycerol (an endocannabinoid) and 2-acetyl monoalkylglycerol ether, a precursor for platelet-activating factor synthesis.
• In adipose tissue and heart, HSL primarily mobilizes stored triglycerides into free fatty acids, while in steroidogenic tissues, it converts cholesteryl esters to free cholesterol for steroid hormone production.
• The protein localizes to the cell membrane, caveolae, cytosol, and lipid droplets, reflecting its dynamic roles in lipid metabolism and signaling.
• HSL is subject to phosphorylation at multiple sites, including Ser660, which regulates its activity and subcellular distribution; it is classified as a phosphoprotein.
• The LIPE gene is alternatively spliced, producing isoforms with distinct tissue distributions; testis shows prominent expression.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to the HSL Ser660 phosphorylation site. For phospho-specific detection, consider treating samples with phosphatase inhibitors during lysis and validate specificity using phosphatase treatment.
• For Western blot (WB), a band at ~117 kDa is expected. Ensure adequate blocking and antibody titration to minimize non-specific binding in complex tissue lysates.
• In ELISA, use a phospho-peptide as a positive control and non-phosphorylated peptide to confirm phosphorylation-state specificity.
• Cross-reactivity with human and mouse HSL is predicted; validate performance in the relevant species model before quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For studies on hormone-sensitive lipase and metabolic disorders, we offer reliable phospho-specific antibodies like this anti-Phospho-HSL-S660 rabbit pAb and can source rare-target reagents for lipid metabolism research. Whether you need raw materials for assay development or production-scale supply, CamelBio supports your project from concept to clinic.