Anti-Phospho-HSF1-S307 Rabbit Polyclonal Antibody for WB, ELISA - Q00613

Core Product Specifications and Parameters

Biological Background: HSF1 Function and Localization

• Heat shock factor protein 1 (HSF1) is a stress-inducible transcription factor that plays a central role in the heat shock response by activating expression of molecular chaperones (HSPs) that protect cells from damage. Related references: PMID:11447121, PMID:12659875, PMID:12917326
• In unstressed cells, HSF1 is maintained in an inactive monomeric state through interaction with an HSP90-containing multichaperone complex. Related references: PMID:11583998, PMID:16278218, PMID:9727490
• Upon stress, HSF1 homotrimerizes and binds to heat shock elements (HSEs) in the promoters of HSP genes to initiate transcription. Related references: PMID:10359787, PMID:11583998, PMID:12659875
• Phosphorylation at Ser307 is a key regulatory modification that modulates HSF1 transcriptional activity and stress response attenuation.
• HSF1 also regulates pre-mRNA processing, nuclear export of HSP70 mRNA, and plays roles in mitosis, DNA repair, and cellular proliferation. Related references: PMID:14707147, PMID:17897941, PMID:18794143, PMID:26359349, PMID:26754925
• Subcellular localization is dynamic: HSF1 is found in the nucleus, cytoplasm, centrosome, and kinetochore depending on the cellular state.
• HSF1 is involved in reactivation of latent HIV-1 by binding to the viral LTR promoter and recruiting transcriptional elongation factors.
• HSF1 undergoes multiple post-translational modifications including phosphorylation, acetylation, and ubiquitination, which regulate its activity.

Experimental Guidance and Technical Tips

• Because this antibody targets the phosphorylation site Ser307, verify phospho-specificity by peptide competition assay or by treating cells with phosphatase inhibitors.
• For WB, a band at approximately 57 kDa is expected. Use heat-shocked human cell lysates (e.g., HeLa or Jurkat) as positive controls; include untreated controls to confirm stress-inducible phosphorylation.
• ELISA: suitable for detecting phospho-HSF1-S307 in human samples. Consider using a capture antibody against total HSF1 and detecting with this phospho-specific antibody for sandwich ELISA.
• Diluents and blocking buffers should be optimized empirically; BSA is generally preferred over milk for phospho-specific antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For heat shock and stress response research, our phospho-HSF1-S307 antibody supports assay development in ELISAs and Western blotting applications. Our one-stop supply chain and technical support help streamline your workflow from concept to clinic.