Anti-Phospho-HP1 gamma/CBX3-S83 Rabbit Polyclonal Antibody for WB, ELISA - Q13185

Core Product Specifications and Parameters

Biological Background: HP1 gamma/CBX3 Function and Localization

• CBX3, also known as HP1 gamma or HECH, is a component of heterochromatin that recognizes and binds histone H3 tails methylated at Lys-9 (H3K9me), contributing to epigenetic repression. Related references: PMID:11242053
• It also binds histone H1.4 when methylated at Lys-26 (H1.4K26me), but is excluded from chromatin when H1.4 is simultaneously phosphorylated at Ser-27 (H1.4S27Ph), indicating a switch mechanism. Related references: PMID:16127177
• CBX3 is involved in kinetochore formation through interaction with the MIS12 complex, playing a role in chromosome segregation. Related references: PMID:15502821
• It mediates the conversion of local chromatin to a repressive heterochromatin-like state via H3K9 trimethylation and recruitment of SUV39H1/2 methyltransferases, particularly in circadian gene regulation (by similarity).
• CBX3 recruits NIPBL to DNA double-strand break sites, facilitating DNA damage repair processes. Related references: PMID:28167679
• Subcellular localization is predominantly nuclear, consistent with its chromatin-associated functions.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, as indicated by keywords; phosphorylation at Ser-83 (the epitope of this antibody) may modulate its activity.
• Alternative names include HECH, HP1-GAMMA, and HP1Hs-gamma, reflecting its identification as a heterochromatin protein 1 family member.

Experimental Guidance and Technical Tips

• Since this antibody is raised against a phospho-peptide around Ser-83, it is expected to specifically recognize the phosphorylated form of HP1 gamma/CBX3. For Western blot, include phosphatase inhibitors in lysis buffers and consider treating samples with phosphatase to confirm phospho-specificity.
• The theoretical molecular weight of CBX3 is ~21 kDa, but migration may vary depending on sample preparation; use appropriate molecular weight markers.
• Validated applications are WB and ELISA; for WB, a typical dilution range may need to be optimized. For ELISA, the antibody can detect phospho-CBX3 in peptide or protein formats.
• If using in immunofluorescence, note that nuclear localization should be observed; co-staining with DNA dyes can aid interpretation.
• Cross-reactivity is reported for human and mouse; for other species, check sequence homology of the immunogen region before use.

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