Anti-Phospho-Histone H3.3 (Ser31) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P84243

Core Product Specifications and Parameters

Biological Background: Histone H3.3 Function and Localization

• Histone H3.3 is a variant histone that replaces conventional H3 in a broad range of nucleosomes in active genes, serving as the predominant H3 form in non-dividing cells.
• Unlike canonical histones, H3.3 is incorporated into chromatin independently of DNA synthesis, marking transcriptionally active regions and maintaining an epigenetic imprint.
• Nucleosomes containing H3.3 play a central role in regulating DNA accessibility, influencing transcription, DNA repair, replication, and chromosomal stability.
• H3.3 undergoes extensive post-translational modifications including acetylation, methylation, and phosphorylation, which constitute the histone code and modulate chromatin structure and gene expression.
• Subcellular localization is restricted to the nucleus, where H3.3 is an integral component of chromosomes.
• The H3.3 protein is encoded by two genes, H3-3A (H3F3A) and H3-3B (H3F3B), both producing identical mature histone proteins.
• Mutations in H3.3 genes have been associated with intellectual disability and other disease phenotypes, highlighting its critical role in neurodevelopment.
• Phosphorylation at serine 31 (S31) is one of the dynamic modifications of H3.3, and the functional consequences are under active investigation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to residues around Ser31 of human H3.3; the antibody is expected to specifically recognize the phosphorylated form, not the unmodified protein.
• For Western blotting, a band around 15 kDa is expected. Include phosphatase inhibitors in lysis buffers to preserve phosphorylation signals.
• For immunohistochemistry (IHC-P) on paraffin sections, antigen retrieval conditions may need optimization; consider testing citrate or EDTA-based methods.
• The predicted wide cross-reactivity suggests potential utility in other species; validate in the desired model system before large-scale use.
• ELISA can be employed for quantitative detection; pair with appropriate secondary antibodies and controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with reliable access to specialized IVD raw materials like this phospho-Histone H3.3 antibody, essential for chromatin and epigenetic studies. Beyond this product, we offer a comprehensive portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents to streamline your assay development. For any rare or custom target needs, our global sourcing network ensures timely delivery from concept to clinic.