Anti-Phospho-Histone H3-T6 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Phospho-Histone H3-T6 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin to regulate transcription, DNA repair, replication, and chromosomal stability.
• Nucleosome structure limits DNA accessibility, and the dynamic regulation of chromatin is controlled by a complex set of post-translational modifications (PTMs), collectively known as the "histone code," including acetylation, methylation, phosphorylation, and others.
• The H3 family includes multiple variants; Q16695 (Histone H3.1t, also known as H3/g or Histone H3.4) is a testis-specific isoform encoded by the H3-4 gene.
• Histone H3.1t localizes to the nucleus and chromosome, consistent with its role in chromatin packaging.
• According to UniProt keywords, H3.1t is subject to diverse PTMs such as acetylation, methylation, phosphorylation, citrullination, ADP-ribosylation, and ubiquitination, which fine-tune chromatin structure and function.
• Tissue specificity: H3.1t is expressed in testicular cells, suggesting a specialized role in male germ cell chromatin.
• Mol weight: ~15.5 kDa.

Experimental Guidance and Technical Tips

• The immunogen is designed around threonine 6 phosphorylation; consider verifying antibody specificity by treating samples with lambda phosphatase or using non-phosphorylated peptide competition.
• For Western blot (WB), a band around 15 kDa is expected; ensure proper nuclear extraction and include both positive and negative controls (e.g., untreated vs. phosphatase-treated cell lysates).
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear staining is anticipated; optimize antigen retrieval and blocking to reduce background.
• The antibody is predicted to react with human, mouse, and rat samples; however, sequence alignment of the immunogen region across species may be necessary for other organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a comprehensive sourcing platform for high-quality IVD raw materials. This anti-Phospho-Histone H3-T6 antibody supports epigenetic and chromatin research applications. Through CamelBio, you can source validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and even rare-target raw materials to accelerate your assay development.