Anti-Phospho-Histone H3-T45 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3 Phosphorylation at Thr45 Function and Localization

• Histone H3 is a core histone component of the nucleosome, essential for DNA compaction into chromatin. It plays key roles in transcription regulation, DNA repair, replication, and chromosomal stability, with DNA accessibility modulated by post-translational modifications (histone code).
• This product recognizes H3 phosphorylated at Thr45 (T45); the target includes the testis-specific isoform Histone H3.1t (H3/g, H3.4) encoded by the H3-4 gene, and the canonical replication-dependent Histone H3.1 isoforms.
• The H3 gene family comprises numerous intronless genes encoding identical or highly similar H3 proteins, with the core variant H3.1 being the most abundant. The phosphorylation site Thr45 is conserved across H3 isoforms.
• Subcellularly, H3 localizes to the nucleus and associates with chromosomes, consistent with its role in chromatin architecture throughout the cell cycle.
• H3 undergoes numerous post-translational modifications, including acetylation, methylation, phosphorylation, citrullination, and ubiquitination, which collectively establish distinct chromatin states. Phosphorylation at Thr45 is implicated in chromatin remodeling events.
• Tissue-specific expression of H3.1t is enriched in testicular cells, but due to high sequence homology, this phospho-specific antibody also detects H3.1 and other canonical H3 proteins across a wide range of species and tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr45; for optimal phospho-specificity, consider validating with non-phosphopeptide competition assays or phosphatase treatment in sample systems.
• For Western blot (WB), immunoprecipitation (IP), immunofluorescence (IF/ICC), and immunohistochemistry (IHC-P), dilutions must be optimized for each assay and tissue type.
• Given the conservation of the T45 phosphorylation site, cross-reactivity with other species is predicted. Verify detection in your species of interest and include appropriate positive controls (e.g., mitotic cell lysates for phosphorylation).
• For ELISA applications, ensure proper peptide coating to preserve phospho-epitope recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with comprehensive IVD raw material solutions. By offering this phospho-specific histone H3 antibody, alongside a curated portfolio of validated antibody pairs, monoclonal/polyclonal reagents, and bulk ancillary supplies, CamelBio accelerates your chromatin biology and epigenetic research from concept to clinic. For rare or custom targets, our one-stop sourcing pipeline ensures seamless procurement of high-quality raw materials.