Anti-Phospho-Histone H3-T11 Rabbit Monoclonal Antibody for WB - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping DNA around histone octamers to form chromatin and regulate DNA accessibility.
• It is essential for transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• H3 is subject to numerous post-translational modifications (e.g., phosphorylation, acetylation, methylation) that constitute the histone code, guiding chromatin dynamics and gene expression.
• The H3 variant encoded by this gene (H3.1t/H3.4) is specifically expressed in testicular cells and localizes to the nucleus/chromosomes.
• H3 undergoes diverse modifications including acetylation, ADP-ribosylation, citrullination, hydroxylation, methylation, phosphorylation, and ubiquitylation.
• The calculated molecular weight of unmodified Histone H3.1t is approximately 15.5 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide designed to specifically recognize Histone H3 phosphorylated at Thr11. Use phosphatase inhibitors during sample preparation to preserve the phosphorylation signal.
• This antibody has been tested in WB, IF-P, IHC-P, ChIP, and ELISA. Optimize dilution and conditions for your specific application and sample type.
• Cross-reactivity has been observed with human, mouse, and rat Histone H3; validate in your species of interest if using other organisms.
• For ChIP, verify antibody performance under your crosslinking and immunoprecipitation conditions, as histone modifications can affect epitope accessibility.
• Store the antibody as recommended (shipped on ice bags) and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing platform for IVD raw materials, providing diagnostic manufacturers and research labs with validated antibodies, antigens, and auxiliary reagents. This phospho-Histone H3 antibody is suited for epigenetics and cancer research applications. Beyond this product, CamelBio offers customized sourcing of rare-target antibodies, bulk reagents, and technical consultation to support your entire development workflow—from concept to clinic.