Anti-Phospho-Histone H3-S28 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3 (Phospho-S28) Function and Localization

• Histone H3 is a core component of the nucleosome, the basic unit of chromatin, and plays a central role in compacting DNA and regulating DNA accessibility.
• Multiple H3 variants exist; Q16695 encodes testis-specific Histone H3.1t (also known as H3/g, H3.4), but the product may recognize other H3 variants including H3.1 (P68431) due to high sequence conservation.
• As part of the nucleosome, H3 contributes to essential nuclear processes such as transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• Histone H3 is subjected to a complex array of post-translational modifications (PTMs), collectively termed the histone code, which modulate chromatin structure and function. Key PTMs include acetylation, methylation, phosphorylation, ubiquitination, and others.
• Phosphorylation at Serine 28 (S28) is part of the histone code and is implicated in chromatin dynamics, influencing mitosis and gene activation.
• Subcellular localization is restricted to the nucleus and associated with chromosomes, consistent with its chromatin-bound role.
• H3.1t shows tissue-specific expression in testicular cells, suggesting specialized functions in spermatogenesis or chromatin remodeling during germ cell development.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide around S28; therefore, it is expected to specifically detect the phosphorylated form of Histone H3. To preserve phosphorylation during sample preparation, include phosphatase inhibitors in lysis buffers.
• For western blotting (WB), the predicted molecular weight is ~15 kDa, and a sharp band should be observed. Consider using nuclear extracts or acid-extracted histones for enriched detection.
• In immunoprecipitation (IP), the rabbit monoclonal format provides high affinity and specificity; validate binding and elution conditions empirically in your target sample.
• For ELISA, the phosphorylated peptide can be used as a positive control; optimize coating and detection conditions to minimize cross-reactivity with non-phosphorylated peptides.
• Cross-reactivity is predicted for a wide range of species based on sequence conservation; however, always verify with appropriate species-specific controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutions a streamlined, one-stop procurement platform for high-quality IVD raw materials, technical support, and consultation—from concept to clinic. This Phospho-Histone H3-S28 antibody supports epigenetics and chromatin research, where validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials are in constant demand. Leverage CamelBio’s supply capabilities to source rare-target raw materials and maintain a reliable inventory for your diagnostic and research workflows.