Anti-Phospho-Histone H3-S10 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3.1t (H3.4) Function and Localization

• Histone H3.1t (also known as H3.4, H3/g, encoded by H3-4/HIST3H3 gene) is a testis-specific core histone variant that replaces canonical histone H3 in nucleosomes during spermatogenesis.
• As a core component of the nucleosome, Histone H3.1t wraps and compacts DNA into chromatin, limiting accessibility to transcription, repair, and replication machineries.
• The H3 gene family includes multiple replication-dependent (such as HIST1H3 and HIST2H3) and replication-independent variants (H3.3), with H3.1t being a tissue-specific member.
• Histone H3.1t localizes to the nucleus and is a component of chromosomal chromatin, where it undergoes extensive post-translational modifications (PTMs) including phosphorylation, acetylation, methylation, and ubiquitination, collectively termed the histone code.
• Phosphorylation of Histone H3 at Serine 10 (H3S10ph) is a key mitotic marker, associated with chromosome condensation, and also plays roles in transcriptional activation of immediate-early genes.
• The tissue specificity of H3.1t to testicular cells suggests a specialized function in chromatin remodeling during spermatogenesis, potentially contributing to male fertility.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phospho-peptide corresponding to Ser10 of human Histone H3. To ensure phosphorylation-specific detection, consider using phosphatase-treated or unstimulated cell lysates as negative controls in Western blotting.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), permeabilization and antigen retrieval steps may be required; optimize fixation and blocking conditions to preserve the phosphorylation epitope.
• The predicted wide species cross-reactivity suggests that the antibody may detect phospho-H3-S10 across vertebrates; however, validation with positive and negative control samples for each species is recommended.
• Given the small molecular weight of Histone H3 (~15 kDa), use high-percentage SDS-PAGE gels (e.g., 15%) and appropriate membrane pore sizes (0.2 µm) for efficient transfer and detection.
• In ELISA and IF applications, titrate the antibody to determine the optimal working dilution, as absolute concentrations have not been empirically determined for this product.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality antibodies and reagents for epigenetic and chromatin research. For targets like phospho-Histone H3, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support assay development. Additionally, our specialized sourcing capabilities can assist in procuring rare-target raw materials, facilitating your transition from concept to clinic.