Anti-Phospho-Histone H3-S10 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3.1t Function and Localization

• Encoded by the H3-4 gene (also known as H3FT, HIST3H3), Histone H3.1t (also designated H3/g or Histone H3.4) is a testis-specific variant of the canonical histone H3.
• As a core histone, H3.1t forms an octamer with H2A, H2B, and H4 to assemble the nucleosome, the fundamental repeating unit of chromatin.
• Nucleosomes wrap and compact DNA, restricting access to DNA-binding proteins and thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• Histone H3.1t localizes to the nucleus and is associated with chromosomes, contributing to chromatin architecture.
• This histone is subject to a complex array of post-translational modifications, including phosphorylation, acetylation, methylation, citrullination, and ubiquitination, which collectively form the 'histone code' that modulates chromatin structure and function.
• Tissue expression is largely confined to testicular cells, suggesting a specialized role in spermatogenesis or chromatin organization in male germ cells.
• The protein has a calculated molecular weight of approximately 15.5 kDa, consistent with the product’s 15 kDa detection weight.

Experimental Guidance and Technical Tips

• As this antibody was raised against a phosphorylated peptide, it is advisable to include both untreated and phosphatase-treated cell lysates in western blotting to confirm phosphorylation-dependent detection.
• For western blotting, a band at approximately 15 kDa corresponds to the predicted molecular weight of histone H3; ensure complete denaturation due to the basic nature of histones.
• In immunofluorescence and immunohistochemistry, nuclear staining is expected; signal may intensify in mitotic cells where histone H3 Ser10 phosphorylation is enriched.
• Cross-reactivity is reported with human, mouse, and rat, and predicted with a wide range of species; nonetheless, it is recommended to validate the antibody in the specific model organism before quantitative assays.
• The antibody can be used for ELISA-based detection of phosphorylated histone H3 peptides; consider using both phosphorylated and non-phosphorylated peptides as controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, supporting developers from early-stage assay design to commercial production. This anti-phospho-Histone H3-S10 monoclonal antibody underscores our commitment to providing high-quality, application-verified reagents for chromatin and cell cycle research. Beyond individual antibodies, we supply matched antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, and can source rare target raw materials to streamline your diagnostic development pipeline.