Anti-Phospho-Histone H2AX-S139 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16104

Core Product Specifications and Parameters

Biological Background: Histone H2AX Function and Localization

• Histone H2AX is a variant histone that replaces conventional H2A in a subset of nucleosomes.
• It plays a crucial role in the DNA damage response, particularly in the repair of double-strand breaks (DSBs), by becoming phosphorylated at Ser139 (forming γ-H2AX) which marks sites of DNA breaks.
• It is required for checkpoint-mediated cell cycle arrest in response to low doses of ionizing radiation.
• As a core histone component, it contributes to chromatin structure, regulating DNA accessibility and playing essential roles in transcription, replication, and chromosomal stability.
• Subcellular localization: H2AX is found in the nucleus and associates with chromosomes.
• Post-translational modifications include acetylation, phosphorylation (notably at S139), and ubiquitination, which regulate its function in DNA repair and chromatin dynamics.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser139; the antibody specifically detects the phosphorylated form. Use phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For Western blot, expect a band at approximately 15 kDa. Verify expression in DNA-damaged samples or cell lines with known DSB induction.
• In IHC-P, antigen retrieval and fixation conditions may affect phospho-epitope integrity; consider optimizing to confirm specificity.
• Cross-reactivity is confirmed for human, mouse, and rat; validate in the appropriate model system for your study.
• Suitable applications include WB, IHC-P, IF/ICC, and ELISA; ensure appropriate controls and antibody dilutions are determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-phospho-Histone H2AX-S139 clone. Our offerings support DNA damage and repair research, from bulk ancillary reagents to rare target sourcing, streamlined for your assay development needs.