Anti-Phospho-Histone H2A-S129 Rabbit pAb - P04911

Core Product Specifications and Parameters

Biological Background: Histone H2A.1 Function and Localization

• Histone H2A.1 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes wrap and compact DNA, thereby regulating DNA accessibility for transcription, replication, repair, and chromosomal stability.
• This histone is critically involved in the DNA damage response, particularly in the repair of DNA double-strand breaks (DSBs) via homologous recombination and non-homologous end joining pathways.
• Post-translational modifications of Histone H2A.1, such as phosphorylation at Ser129 (equivalent to the mammalian H2AX S139), acetylation, methylation, and ubiquitination, form a histone code that modulates chromatin structure and recruits repair proteins to damage sites.
• The protein is exclusively localized to the nucleus and is associated with chromosomes throughout the cell cycle.
• S. cerevisiae Histone H2A.1 is encoded by the HTA1 gene and belongs to the H2A histone family, which includes highly conserved variants across eukaryotes.
• Keywords associated with P04911 include DNA damage, DNA repair, phosphoprotein, nucleosome core, and Ubl conjugation, underscoring its key roles in genome maintenance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to phosphorylated Ser129 of yeast Histone H2A. Therefore, the antibody specifically recognizes the phosphorylated form; ensure that experimental conditions preserve phosphorylation status (e.g., use phosphatase inhibitors during sample preparation).
• For IHC-P, antigen retrieval methods and tissue fixation protocols should be optimized for phosphorylated epitopes. Consider testing citraconic anhydride or heat-induced epitope retrieval in citrate buffer (pH 6.0) if standard methods yield weak staining.
• In ELISA, the antibody may be used for detection of phosphorylated H2A peptides or proteins. Verify cross-reactivity with the endogenous target in the relevant species (human, rat) by including appropriate controls and blocking with non-phosphorylated peptides.
• Although the immunogen is derived from yeast, cross-reactivity is reported for human and rat. However, sequence alignment reveals that the region around Ser129 is highly conserved in higher eukaryotes; still, validation in the specific sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive portfolio of IVD raw materials. For chromatin biology and DNA repair research, we offer a range of validated antibody pairs, purified monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our sourcing expertise extends to rare targets like phospho-specific histone antibodies, ensuring you can procure the critical components needed from concept to clinic.