Anti-Phospho-HER2/ErbB2 (Y1221/1222) Rabbit Polyclonal Antibody for WB, ELISA - P04626

Core Product Specifications and Parameters

Biological Background: HER2/ErbB2 Function and Localization

• Receptor tyrosine-protein kinase erbB-2, also known as HER2, NEU, or CD340, is encoded by the ERBB2 gene and functions as a cell surface receptor that requires a coreceptor for ligand binding, forming essential neuregulin-receptor complexes.
• HER2 regulates microtubule stability and outgrowth via a MEMO1-RHOA-DIAPH1 pathway that inhibits GSK3B, allowing membrane association of APC and MACF1 for microtubule capture.
• In the nucleus, HER2 acts as a transcriptional regulator; it binds the PTGS2/COX-2 promoter at the 5'-TCAAATTC-3' sequence and activates transcription, and is implicated in CDKN1A transcriptional activation involving STAT3 and SRC.
• HER2 also enhances protein synthesis by promoting rRNA gene transcription through RNA Pol I, linking cell surface signaling to ribosome biogenesis.
• Localization includes the cell membrane, ruffle membrane, early endosomes, cytoplasm (perinuclear region), and nucleus, reflecting its dynamic signaling roles.
• Post-translational modifications include phosphorylation at multiple tyrosine residues (e.g., Y1221/1222) that regulate kinase activity and downstream signaling, as well as glycosylation and disulfide bonds.
• Expressed in various tumor tissues, including primary breast tumors and carcinomas of the small bowel, esophagus, kidney, and mouth, highlighting its role as an oncoprotein and therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y1221 and Y1222, suggesting the antibody specifically recognizes the dual-phosphorylated form of HER2; consider phosphatase inhibitor treatment during sample preparation to preserve phosphorylation.
• For Western blot (WB), validate the antibody in cell lines or tissues with known HER2 Y1221/1222 phosphorylation, such as heregulin-stimulated cells or tumor samples; a band near 138 kDa is expected.
• In ELISA applications, the antibody may be used to detect or quantify phosphorylated HER2 in lysates or as a capture/detection reagent; optimize coating and blocking conditions empirically.
• Cross-reactivity is reported for human species only; for non-human samples, verify sequence homology of the phosphopeptide region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD manufacturers, offering a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This phosphorylated HER2 antibody supports oncology and cell signaling research, and CamelBio’s supply expertise ensures reliable access to rare-target raw materials and customized sourcing solutions. Let us streamline your procurement needs from concept to clinic.