Anti-Phospho-HDAC4-S246 + HDAC5-S259 + HDAC9-S220 Rabbit Monoclonal Antibody - P56524 / Q9UQL6 / Q9UKV0

Core Product Specifications and Parameters

Biological Background: HDAC4/5/9 Function and Localization

• Histone deacetylase 4 (HDAC4) is a class II histone deacetylase that catalyzes the removal of acetyl groups from lysine residues on the N-terminal tails of core histones (H2A, H2B, H3, H4), leading to chromatin compaction and epigenetic transcriptional repression.
• HDAC4 acts as a transcriptional repressor through the formation of large multiprotein complexes and is critically involved in cell cycle progression and developmental events.
• Through direct interaction with myocyte enhancer factor-2 (MEF2) family members (MEF2A, MEF2C, MEF2D), HDAC4 plays a key role in muscle maturation and differentiation.
• HDAC4 participates in the MTA1-mediated epigenetic regulation of estrogen receptor 1 (ESR1) expression in breast cancer, linking deacetylase activity to hormone signaling.
• HDAC4 deacetylates HSPA1A and HSPA1B at Lys-77, promoting their preferential binding to the co-chaperone STUB1 (PubMed:27708256). Related references: PMID:27708256
• The protein localizes to both the nucleus and cytoplasm, shuttling in response to phosphorylation signals; it is ubiquitously expressed across tissues.
• HDAC4 is subject to extensive post-translational modifications (phosphorylation, ubiquitination) and is associated with neurodevelopmental disorders including epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide spanning S246 of HDAC4, S259 of HDAC5, and S220 of HDAC9; antibody specificity for phosphorylated forms should be confirmed by phosphatase treatment of lysates.
• For Western blot, nuclear and cytoplasmic fractions may both yield bands due to HDAC4 shuttling; observed molecular weights can range from 57–65 kDa (possibly cleaved/isoform) to 97–122 kDa (full-length). Validate with positive and negative controls.
• In IHC-P, antigen retrieval conditions may need optimization; consider including phospho-specific blocking peptides to confirm staining.
• Cross-reactivity with mouse and rat HDAC4/5/9 is indicated; however, sequence alignment of target phospho-sites should be verified if used in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This phospho‑HDAC4/5/9 rabbit monoclonal antibody supports epigenetic and signal transduction research applications. Through its integrated procurement model, CamelBio also facilitates customized sourcing of rare‑target raw materials to accelerate your assay development pipeline.