Anti-Phospho-HDAC2 (S394) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q92769

Core Product Specifications and Parameters

Biological Background: HDAC2 Function and Localization

• HDAC2 (Histone deacetylase 2) is a chromatin-modifying enzyme that catalyzes the deacetylation of lysine residues on core histones (H2A, H2B, H3, H4), leading to epigenetic repression and transcriptional silencing. Related references: PMID:28497810

• It functions within large multiprotein complexes, such as NuRD, SIN3B, and RCOR/GFI/KDM1A/HDAC, which recruit HDAC activity to specific genomic regions to regulate transcription, cell cycle progression, and development. Related references: PMID:12724404 PMID:16428440 PMID:28977666 PMID:37137925

• Beyond histones, HDAC2 deacetylates non-histone targets such as TSHZ3 and MTA1, modulating their transcriptional repressor activities on genes like TFF1 and CDKN1A. Related references: PMID:19343227 PMID:21965678

• HDAC2 also acts as a protein-lysine deacylase, removing crotonyl, lactyl, and 2-hydroxyisobutyryl groups from lysine residues, expanding its regulatory capacity beyond acetylation. Related references: PMID:28497810 PMID:29192674 PMID:35044827

• The protein localizes to both the nucleus and cytoplasm, consistent with roles in chromatin modulation and potential cytoplasmic signaling functions.

• HDAC2 is widely expressed across tissues, with relatively lower levels observed in brain and lung.

• Phosphorylation at serine 394 represents a key post-translational modification that may regulate HDAC2 activity, interactions, or localization; additional modifications include acetylation, S-nitrosylation, and ubiquitination.

Experimental Guidance and Technical Tips

• As a phospho-specific antibody raised against a peptide surrounding Ser394, include appropriate controls such as phosphatase-treated lysates or non-phosphopeptide blocking to confirm signal specificity.
• For Western blotting, a band around 55 kDa is expected; use phospho-protective lysis and running buffers, and validate detection with positive and negative control samples.
• In IHC-P applications, optimize fixation (e.g., 4% PFA) and antigen retrieval to preserve phospho-epitopes; validate staining with isotype controls and competitive inhibition.
• In ELISA, ensure efficient coating of the target peptide and assess cross-reactivity with the corresponding non-phosphorylated peptide to verify phospho-specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with streamlined access to a wide range of IVD raw materials, including this phospho-HDAC2 antibody for epigenetic and transcriptional regulation studies. Our portfolio extends to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare or custom targets—supporting your assay development from concept to clinic.