Anti-Phospho-GYS1-S641 Rabbit Polyclonal Antibody for WB, ELISA - P13807

Core Product Specifications and Parameters

Biological Background: Glycogen Synthase 1 (GYS1) Function and Localization

• GYS1 encodes the muscle isoform of glycogen synthase, a key enzyme in glycogen biosynthesis; alternative splicing generates multiple isoforms (keyword: Alternative splicing).
• Glycogen synthase works cooperatively with glycogenin and glycogen branching enzyme to elongate the glycogen primer by transferring glucose from UDP-glucose to the non-reducing end of alpha-1,4-glucan chains.
• The enzyme is allosterically regulated and undergoes phosphorylation at multiple sites, including Ser641, modulating its activity in response to insulin and metabolic signals (keywords: Allosteric enzyme, Phosphoprotein).
• Predominantly expressed in skeletal muscle, GYS1 is also found in other glycogen-containing tissues, playing a central role in glucose storage and energy homeostasis.
• Mutations in GYS1 are associated with glycogen storage disease and diabetes mellitus, highlighting its clinical significance (keywords: Disease variant, Diabetes mellitus).
• As a glycosyltransferase family member, GYS1 catalyzes the formation of alpha-1,4-glycosidic bonds, a critical step in glycogen synthesis.

Experimental Guidance and Technical Tips

• Since this antibody targets a phosphorylation site, ensure that GYS1 is phosphorylated under experimental conditions; include phosphatase inhibitors during sample preparation to preserve the phospho-signal.
• For WB, expect a band at approximately 84 kDa; validate phospho-specificity by treating samples with lambda phosphatase or using a non-phospho peptide blocking control.
• For ELISA, employ the phospho-peptide as a positive control and the corresponding non-phosphorylated peptide to assess binding specificity.
• Cross-reactivity is confirmed for human, mouse, and rat; if used in other species, verify the conservation of the Ser641 flanking sequence by alignment.
• Optimal working dilutions for WB and ELISA should be determined empirically; start with the manufacturer’s recommendation and titrate according to the sample system.
• Use blocking buffers without phosphate to minimize potential non-specific interactions with phospho-epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. For glycogen metabolism investigations, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our global sourcing network ensures reliable supply of specialized targets like phospho-specific GYS1 antibodies, supporting your assay development from concept to clinic.