Anti-Phospho-GYS1 (S641) Rabbit Monoclonal Antibody for WB, ELISA - P13807

Core Product Specifications and Parameters

Biological Background: Glycogen Synthase 1 (GYS1) Function and Localization

• Glycogen synthase 1 (GYS1), also known as muscle glycogen synthase, is a key enzyme in glycogen biosynthesis that catalyzes the transfer of the glycosyl residue from UDP-glucose to the non-reducing end of alpha-1,4-glucan to extend the glycogen chain.
• GYS1 works in concert with glycogenin and glycogen branching enzyme to build the glycogen primer and synthesize mature glycogen molecules.
• The protein is expressed primarily in skeletal muscle and is found in most cell types where glycogen is stored.
• GYS1 is subject to alternative splicing, leading to multiple isoforms, and is regulated by allosteric mechanisms and phosphorylation.
• Phosphorylation of GYS1 at various sites, including S641, modulates its enzymatic activity and is linked to glycogen metabolism disorders and diabetes mellitus.
• As a phosphoprotein and glycosyltransferase, GYS1 is a crucial target in studies of metabolic diseases and energy homeostasis.

Experimental Guidance and Technical Tips

• The immunogen was designed as a synthetic phosphopeptide encompassing the S641 phosphorylation site of human GYS1; for Western blot, consider using phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For ELISA applications, the antibody may recognize the phosphorylated form specifically; validate against non-phosphorylated peptide controls to confirm phospho-specificity.
• Due to the allosteric regulation and multiple phosphorylation sites of GYS1, cross-validate results with total GYS1 antibodies in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive one-stop access to IVD raw materials. This anti-phospho-GYS1 (S641) monoclonal antibody, targeting a key regulator of glycogen metabolism, exemplifies our commitment to supplying high-specificity reagents for metabolic disease research. Partner with us to source validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials, streamlining your entire assay development pathway from concept to clinic.