Anti-Phospho-GluR1/GRIA1-S849 Rabbit Polyclonal Antibody for WB, ELISA - P42261

Core Product Specifications and Parameters

Biological Background: Glutamate receptor 1 (GRIA1) Function and Localization

• GRIA1 (Glutamate receptor 1), also known as GluR1, GluA1, or AMPA-selective glutamate receptor 1, is a ligand-gated ion channel belonging to the AMPA receptor subfamily of ionotropic glutamate receptors.
• It functions as a cation channel gated by L-glutamate and AMPA receptor agonists, mediating fast excitatory synaptic transmission; upon activation, it allows influx of Na+ and Ca2+, leading to membrane depolarization. The receptor undergoes rapid desensitization, and resensitization dynamics are modulated by auxiliary subunits such as CACNG2/4/7/8 and CNIH2. Related references: PMID:1311100, PMID:20805473, PMID:21172611
• Calcium permeability is determined by subunit composition; heteromeric channels containing edited GRIA2 are calcium-impermeable.
• Subcellularly, GRIA1 is localized to the postsynaptic density membrane, dendritic spines, as well as endoplasmic reticulum, early and recycling endosomes, and the presynaptic compartment.
• It is widely expressed in brain, and is subject to post-translational modifications including phosphorylation, palmitoylation, glycosylation, and disulfide bond formation, which regulate its trafficking and function.
• Mutations in GRIA1 are associated with intellectual disability and neurological disorders, underscoring its critical role in synaptic function.

Experimental Guidance and Technical Tips

• For Western blot, use phospho-specific controls (e.g., phosphatase-treated lysates) to confirm band specificity at the expected molecular weight of approximately 102 kDa.
• Optimize antibody dilution and blocking conditions empirically; test phospho-peptide competition to verify signal dependence.
• This antibody is suitable for ELISA detection of phospho-GluR1 peptides; consider paired non-phospho antibody for normalization.
• Cross-reactivity to mouse and rat is indicated; validate in relevant brain tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing solutions, offering this anti-phospho-GluR1 antibody along with a broad portfolio of validated antibody pairs, recombinant antigens, and ancillary reagents for neuroscience-focused diagnostic development. Our platform simplifies procurement from concept to clinic, supporting rare target sourcing and custom antibody services to accelerate your assay development pipeline.