Anti-Phospho-GCN2 (T899) Rabbit Polyclonal Antibody for WB, ELISA - Q9P2K8

Core Product Specifications and Parameters

Biological Background: eIF-2-alpha kinase GCN2 Function and Localization

• EIF2AK4 (eIF-2-alpha kinase GCN2) is a serine/threonine-protein kinase and phosphoprotein that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability. Related references: PMID:25329545 PMID:32610081
• Acting as a sensor of amino acid depletion, GCN2 activates the integrated stress response (ISR), leading to global translational repression and selective translation of ATF4 to reprogram cellular metabolism. Related references: PMID:32610081
• GCN2 mediates cell cycle arrest under stress by repressing cyclin D1 mRNA translation and promoting CDKN1A/p21 mRNA translation. Related references: PMID:26102367
• During yellow fever virus infection, GCN2 promotes dendritic cell autophagy and antigen presentation to CD4+ and CD8+ T cells under amino acid starvation, modulating adaptive immunity. Related references: PMID:24310610
• Exhibits antiviral activity against alphaviruses by inhibiting early viral mRNA translation of incoming genomic RNA, thereby restricting replication.
• Plays a role in synaptic plasticity, learning, long-term memory formation, neurite outgrowth inhibition, and promotes apoptosis upon glucose deprivation.
• Localizes predominantly to the cytoplasm.
• Widely expressed across tissues, with notable expression in lung, smooth muscle cells, and macrophages.

Experimental Guidance and Technical Tips

• For Western blotting, include phosphatase inhibitors in the lysis buffer to preserve phosphorylation at T899.
• Validate the antibody in relevant human cell lines subjected to amino acid starvation or endoplasmic reticulum stress to induce GCN2 phosphorylation.
• In ELISA, use a non-phosphorylated peptide as a negative control and perform peptide competition assays to confirm phospho-specificity.
• Optimal antibody dilutions should be determined experimentally for each assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this Phospho-GCN2 (T899) antibody along with a broad portfolio of kinases and stress response targets. Our supply capabilities include validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to support assay development. With expertise in rare and challenging targets, CamelBio enables streamlined procurement from initial research to clinical application.