Anti-Phospho-GAB1 (Y307) Rabbit Polyclonal Antibody for WB, ELISA - Q13480

Core Product Specifications and Parameters

Biological Background: GAB1 Function and Localization

• GRB2-associated-binding protein 1 (GAB1) is a multisite adapter protein that integrates signals from multiple receptor tyrosine kinases, including EGFR, insulin receptor (INSR), and MET.
• GAB1 plays a crucial role in FGFR1 signaling and is involved in the MET/hepatocyte growth factor (HGF) pathway, contributing to cell proliferation, survival, and migration.
  Related references: PMID:29408807
• Alternative splicing generates multiple isoforms of GAB1, potentially modulating its interaction networks and signaling specificity.
• GAB1 is a member of the GAB family of scaffold proteins (GAB1, GAB2, GAB3) that share a conserved pleckstrin homology (PH) domain and proline-rich regions for SH3 domain interactions.
• The protein is regulated by post-translational modifications, including phosphorylation (e.g., Y307, critical for recruitment of downstream effectors) and acetylation, influencing its signaling competence.
• Mutations in GAB1 are associated with DFNB26, a form of autosomal recessive nonsyndromic deafness, highlighting a role in auditory system development or function.

Experimental Guidance and Technical Tips

• Use standard WB lysate preparation with phosphatase inhibitors to preserve phosphorylation at Y307; consider treating samples with lambda phosphatase as a negative control to confirm signal specificity.
• For ELISA, the phospho-peptide immunogen can be used as a coating antigen to validate antibody titer; competitive ELISA with non-phosphorylated peptide may help assess site-specificity.
• Given cross-reactivity with mouse, validate the antibody in mouse cell lines or tissues stimulated with appropriate growth factors (e.g., HGF, EGF) to assess species reactivity.
• The predicted molecular weight is 77 kDa; however, multiple isoforms and post-translational modifications may result in additional bands; confirm with specific GAB1 siRNA or knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering manufacturers and researchers streamlined access to high-quality antibodies, antigens, and technical support from concept to clinic. For phospho-specific targets like GAB1-Y307, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to your signal transduction assay development needs. Leverage our expertise to secure rare targets and accelerate your diagnostic or research program.