Anti-Phospho-Fyn-S21 Rabbit Polyclonal Antibody for WB, ELISA - P06241

Core Product Specifications and Parameters

Biological Background: Tyrosine-protein kinase Fyn Function and Localization

• FYN encodes a non-receptor tyrosine kinase of the Src family, also known as Proto-oncogene Syn, c-Fyn, Src-like kinase (SLK), and p59-Fyn, containing SH2 and SH3 domains.
• FYN is a non-receptor tyrosine-protein kinase that regulates cell growth, survival, adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response, and axon guidance. Related references: PMID:11536198, PMID:15489916, PMID:15557120
• It modulates cell adhesion and motility by phosphorylating CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Related references: PMID:17194753
• Cytoskeletal remodeling is mediated through phosphorylation of actin regulator WAS and microtubule-associated proteins MAP2 and MAPT. Related references: PMID:14707117, PMID:15536091
• In T-cells, FYN participates in TCR signaling, phosphorylating PTK2B/PYK2, VAV1, and mediating negative feedback via PAG1 and PDCD1. Related references: PMID:22080863, PMID:20028775, PMID:18056706, PMID:32184441, PMID:11005864
• Neuronal functions include phosphorylation of DPYSL2, ARHGAP32, and SNCA, contributing to axon guidance and synaptic regulation. Related references: PMID:11162638, PMID:12788081, PMID:19652227
• In the kidney, it maintains glomerular filtration by phosphorylating slit diaphragm proteins NPHS1, KIRREL1, and TRPC6. Related references: PMID:14761972, PMID:18258597, PMID:19179337
• Subcellularly, FYN localizes to the cytoplasm, nucleus, cell membrane, and perikaryon; isoform 1 is highly expressed in brain, while isoform 2 predominates in T-lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues around serine 21 (S21) of human Fyn (NP_002028.1); consider verifying phospho-specificity using non-phosphorylated peptide controls.
• For Western blotting, include phosphatase inhibitors during sample preparation and validate band authenticity by lambda phosphatase treatment.
• In ELISA, test parallel wells coated with phosphorylated and non-phosphorylated peptides to confirm selective binding.
• Cross-reactivity with species other than human has not been evaluated; perform species-specific validation if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-Phospho-Fyn-S21 antibody supports research in kinase signaling, immune regulation, and neural development. From rare-target procurement to custom production, CamelBio bridges your needs from concept to clinic.