Anti-Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q12778 / O43524 / P98177

Core Product Specifications and Parameters

Biological Background: FOXO1/FOXO3A/FOXO4 Function and Localization

• FOXO1 (Forkhead box O1) is a transcription factor and a key member of the FOXO family, which also includes FOXO3A and FOXO4—all three are targets of this antibody.
• FOXO1 is the main mediator of insulin signaling, binding the insulin response element (IRE) to regulate metabolic homeostasis and redox balance in response to oxidative stress. Related references: PMID:10358076 PMID:12228231 PMID:15220471
• In hepatocytes, FOXO1 promotes gluconeogenesis by activating genes such as G6PC1 and PCK1, working in concert with coactivators like PPARGC1A. Related references: PMID:17024043
• FOXO1 is a critical regulator of programmed cell death, functioning downstream of kinases (e.g., AKT1, STK4) and promoting apoptosis in neurons, cardiac smooth muscle cells, and endothelial cells. Related references: PMID:18356527 PMID:19221179 PMID:19483080 PMID:31063815
• Under starvation or oxidative stress, FOXO1 induces autophagy in a transcription-independent manner. Related references: PMID:20543840
• FOXO1 dynamically shuttles between the cytoplasm and nucleus; its subcellular localization and transcriptional activity are tightly controlled by post‑translational modifications, notably phosphorylation at Thr‑24 (and analogous sites in FOXO3A/FOXO4) by AKT, which drives nuclear exclusion.
• FOXO1 is abundantly expressed in skeletal muscle and ovary, with moderate levels in heart, placenta, lung, liver, pancreas, and other tissues; it is also detected in endothelial cells. Related references: PMID:9479491 PMID:19483080

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to the Thr‑24 region of human FOXO1. Based on sequence homology, it is predicted to also recognize the phosphorylated Thr‑32 of FOXO3A and Thr‑28 of FOXO4.
• For Western blot analysis, include phosphatase inhibitors in the lysis buffer to preserve the phosphorylation signal. A band near 70 kDa is expected for FOXO1, with similar sizes for FOXO3A and FOXO4.
• When performing immunohistochemistry (IHC‑P), consider validating the staining in tissues known to express FOXO1 (e.g., skeletal muscle, liver) and using a dephosphorylated control to confirm phospho‑specificity.
• ELISA applications may benefit from using the immunogen peptide as a positive control to assess detection sensitivity.
• As with all phospho‑specific antibodies, pre‑absorption of the antibody with the non‑phosphorylated peptide can be used to verify specificity.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of high‑quality IVD raw materials. This anti‑phospho‑FOXO1/3A/4 antibody is well‑suited for developing assays in metabolic disease, oncology, and cell signaling research. Beyond phospho‑specific reagents, we supply validated antibody pairs, bulk ancillary reagents, and offer customized sourcing for rare or challenging targets—providing a seamless path from concept to clinic.