Anti-Phospho-FGFR1 (Y653/Y654) Rabbit Polyclonal Antibody for WB, ELISA - P11362

Core Product Specifications and Parameters

Biological Background: FGFR1 Function and Localization

• FGFR1 is a cell-surface tyrosine-protein kinase receptor for fibroblast growth factors, essential for embryonic development, cell proliferation, differentiation, and migration.
• Ligand binding triggers activation of multiple signaling cascades including PLCG1, RAS-MAPK (ERK), and AKT1 pathways, regulating transcription and cell fate.
• The receptor localizes primarily to the cell membrane but also traffics to the nucleus, cytoplasm, and cytoplasmic vesicles, contributing to signaling diversity.
• FGFR1 expression is detected in astrocytoma, neuroblastoma, and adrenal cortex cell lines; certain isoforms are present in foreskin fibroblasts.
• Post-translational modifications include phosphorylation at tyrosine residues Y653 and Y654 in the activation loop, critical for kinase activity and downstream signaling.
• FGFR1 is implicated in multiple diseases including craniosynostosis, dwarfism, and Kallmann syndrome, often due to gain-of-function mutations or chromosomal rearrangements.
• The receptor is a glycoprotein with heparin-binding properties and undergoes ubiquitination and internalization for signal down-regulation.

Experimental Guidance and Technical Tips

• Use phosphatase inhibitors during cell lysis and sample preparation to preserve the phosphorylation status of FGFR1.
• Include a negative control (e.g., lambda phosphatase-treated lysate) to confirm phospho-specificity in Western blot.
• Optimize antibody dilution for your specific assay; typical starting dilutions for WB are 1:500–1:2000, but must be determined empirically.
• Validate cross-reactivity with mouse FGFR1 in relevant mouse tissues or cell lines before large-scale experiments.
• For ELISA, consider using a phospho-peptide blocking step to ensure specificity for the phosphorylated epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including this anti-phospho-FGFR1 (Y653/Y654) polyclonal antibody for cancer and developmental biology research. Our comprehensive portfolio features validated antibody pairs, bulk ancillary reagents, and rare-target sourcing, backed by technical consulting to support every stage from concept to clinic. Partner with CamelBio to accelerate your assay development with reliable, one-stop supply solutions.