Anti-Phospho-FGFR1 (Y653) Rabbit Polyclonal Antibody for WB, ELISA - P11362

Core Product Specifications and Parameters

Biological Background: FGFR1 Function and Localization

• FGFR1 is a receptor tyrosine kinase that binds fibroblast growth factors, triggering intracellular signaling cascades crucial for cell proliferation, differentiation, and migration.
• It plays a pivotal role in embryonic development, including mesoderm patterning, axial organization, skeletogenesis, and GnRH neuronal system development.
• Upon ligand binding, FGFR1 activates downstream pathways such as RAS-MAPK and AKT1, and phosphorylates substrates like PLCG1, FRS2, and SHC1.
• The protein localizes to the cell membrane, nucleus, cytosol, and cytoplasmic vesicles, reflecting its dynamic role in signal transduction and transcriptional regulation.
• FGFR1 undergoes auto-phosphorylation at tyrosine residues (e.g., Y653 in the kinase domain) critical for full kinase activation and downstream signaling.
• Alternative splicing generates multiple isoforms with tissue-specific expression, detected in astrocytoma, neuroblastoma, and adrenal cortex cell lines.
• Post-translational modifications include phosphorylation, ubiquitination, and glycosylation, which regulate its activity, stability, and cellular trafficking.
• Mutations and chromosomal rearrangements in FGFR1 are linked to developmental disorders and cancers, highlighting its importance in disease.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to phosphorylated Y653 of human FGFR1. Verify phospho-specificity by testing with non-phosphorylated peptide or phosphatase-treated samples.
• For WB, a band around 92 kDa is expected; use appropriate loading controls and confirm expression in relevant cell lines (e.g., FGFR1-positive human, mouse, or rat samples).
• For ELISA, the phosphorylated peptide may be used as a standard, and cross-reactivity with other phosphorylated FGFRs should be assessed.
• Include blocking with BSA to reduce nonspecific binding, and optimize antibody dilution for your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, antigens, and ancillary reagents to support diagnostic development. This anti-Phospho-FGFR1 (Y653) antibody is part of our growing portfolio of phosphorylation-state-specific antibodies for cell signaling research and assay development. We also supply bulk quantities, custom antibody pairs, and rare-target sourcing to streamline your workflow from concept to clinic.