Anti-Phospho-FAK-Y925 Rabbit Polyclonal Antibody for WB, ELISA - Q05397

Core Product Specifications and Parameters

Biological Background: FAK Function and Localization

• Focal adhesion kinase 1 (FAK/PTK2) is a non-receptor tyrosine kinase that serves as a critical regulator of cell migration, adhesion, spreading, and actin cytoskeleton reorganization. It integrates signaling from integrins, growth factor receptors, and G protein-coupled receptors.
• FAK undergoes autophosphorylation at Y397 upon activation, creating a high-affinity binding site for SRC family kinases. Subsequent phosphorylation at Y925 promotes GRB2 recruitment, linking to the MAP kinase/ERK signaling cascade and contributing to cell proliferation and survival.
• The protein localizes dynamically to focal adhesions, the cell membrane, perinuclear cytoplasm, cell cortex, centrosome, and nucleus. This distribution allows spatiotemporal control of signal transduction and cytoskeletal remodeling.
• FAK phosphorylates multiple substrates, including NEDD9 (enhancing integrin-mediated signaling), BCAR1, GRB7, and SRC itself, thereby modulating downstream pathways such as PI3K/AKT and Rho GTPase cascades. Related references: PMID:9360983
• Isoform 6 (FRNK), an alternatively spliced variant lacking the kinase domain, acts as an endogenous inhibitor by blocking FAK phosphorylation and nuclear signaling, attenuating SRF-dependent transcription.
• FAK is ubiquitously expressed, with notable detection in B and T lymphocytes, lung fibroblasts, and epithelial cells. Related references: PMID:31630787
• Post-translational modifications, especially phosphorylation at residues Y397, Y576, Y577, Y861, and Y925, tightly regulate FAK activity. Y925 phosphorylation specifically drives interaction with GRB2 and MAPK pathway activation, making it a key marker for FAK signaling in developmental and cancer contexts.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide surrounding tyrosine 925 of human FAK; the antibody is expected to recognize FAK only when phosphorylated at this site. For optimal specificity in WB, consider using phosphatase inhibitors during sample preparation to preserve phosphorylation.
• Predicted molecular weight: ~119 kDa. A band near this size in human cell lysates supports detection, but additional bands from isoforms or post-translational modifications may occur, so compare with total FAK controls.
• For ELISA, include a non-phosphorylated peptide control to confirm phospho-specificity. Titrate antibody concentration to minimize background and maximize signal-to-noise ratio in your specific assay conditions.
• This product has documented reactivity with human samples; cross-reactivity with other species is unknown. Validate performance in any non-human model system with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material procurement, connecting diagnostic manufacturers and research labs with validated antibodies like this phospho-FAK-Y925 pAb for cytoskeletal and adhesion studies. Our global network ensures access to optimized monoclonal/polyclonal antibodies, matched antibody pairs, and ancillary bulk reagents to streamline assay development. For rare or custom targets in cell signaling, CamelBio offers tailored sourcing and technical support to bridge your concept-to-clinic pipeline.