Anti-Phospho-FAK-Y576/Y577 Rabbit Monoclonal Antibody for WB, ELISA - Q05397

Core Product Specifications and Parameters

Biological Background: FAK (Focal Adhesion Kinase 1) Function and Localization

• FAK (PTK2) is a non-receptor protein-tyrosine kinase encoded by the PTK2 gene; isoform 6 (FRNK) lacks the kinase domain and acts as an endogenous inhibitor of FAK signaling.
• It plays a central role in integrin-mediated signal transduction, regulating cell adhesion, spreading, migration, and cytoskeletal reorganization at focal adhesions.
• Upon activation, FAK forms signaling complexes with SRC family kinases, leading to phosphorylation of multiple downstream effectors including CAS family members, and promoting MAP kinase and PI3K/AKT pathways.
• Specifically, FAK phosphorylates NEDD9 following integrin stimulation, facilitating downstream effects on cell motility. Related references: PMID:9360983
• Subcellularly, FAK localizes to focal adhesions, the cell cortex, perinuclear cytoplasm, centrosomes, and the nucleus, reflecting its dynamic roles in signaling from the membrane to the nucleus.
• FAK is ubiquitously expressed, with detection in B- and T-lymphocytes, fibroblasts, and epithelial cells (PubMed:31630787). Related references: PMID:31630787
• Post-translational modifications include phosphorylation on multiple tyrosine residues (such as Y576/Y577 for activation), acetylation, and ubiquitination, which modulate its kinase activity, scaffolding functions, and protein stability.
• Key biological processes involving FAK include embryonic development, angiogenesis, neuronal migration, and osteoblast differentiation, highlighting its importance beyond cell adhesion.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide corresponding to residues surrounding Y576 and Y577 of human FAK. For Western blot, consider using a positive control such as lysates from cells stimulated with fibronectin or growth factors to induce FAK phosphorylation.
• To preserve phosphorylation signals, include phosphatase inhibitors in lysis buffers and avoid excessive sample handling.
• The antibody cross-reacts with mouse FAK, allowing its use in mouse model studies; however, validation in the specific sample system is recommended.
• For ELISA applications, coating with a phospho-peptide antigen may serve as a positive control to assess antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, supporting diagnostic manufacturers and research laboratories with validated antibodies, antigen pairs, and ancillary reagents. This anti-phospho-FAK antibody is well-suited for researchers studying cell adhesion, migration, and cancer signaling pathways. Beyond this product, CamelBio offers custom sourcing of rare-target reagents and bulk supply options to accelerate your assay development and clinical translation.