Anti-Phospho-ERK1-Y204 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P27361

Core Product Specifications and Parameters

Biological Background: ERK1 Function and Localization

• Protein identity and isoforms: Mitogen-activated protein kinase 3 (MAPK3), commonly known as ERK1 or p44-MAPK, is a member of the serine/threonine kinase family. Alternative splicing of the MAPK3 gene gives rise to multiple isoforms.
• Core MAPK/ERK cascade function: ERK1 acts as an essential component of the MAP kinase signal transduction pathway, mediating diverse biological functions such as cell growth, adhesion, survival, and differentiation through the regulation of transcription, translation, and cytoskeletal rearrangements. Related references: PMID:34497368
• Coordination with ERK2: MAPK3/ERK1 and MAPK1/ERK2 are the two principal MAPKs that drive the MAPK/ERK cascade, also participating in signaling initiated by activated KIT and KITLG/SCF.
• Extensive substrate network: ERK1 phosphorylates approximately 160 substrates, including transcription factors, cytoskeletal elements, apoptosis regulators, and translation regulators, thereby coordinating a wide spectrum of cellular processes. Related references: PMID:35216969
• Subcellular localization: Found in the cytoplasm, nucleus, membrane, caveolae, and focal adhesions, enabling signal propagation across multiple cellular compartments.
• Key post-translational modifications: The antibody targets phosphorylation at Y204, a critical activation site. ERK1 is also subject to acetylation, ubiquitin conjugation, and other modifications that modulate its stability and function.
• Functional keywords: Keywords associated with P27361 include ATP-binding, kinase, serine/threonine-protein kinase, phosphoprotein, nucleus, cytoplasm, cell cycle, apoptosis, and host-virus interaction, reflecting its central role in cell signaling.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y204 of human ERK1. Target specificity for the phosphorylated form should be confirmed, especially if ERK2 isoforms are present, by using non-phosphorylated peptide controls or phosphatase treatment.
• For western blotting, include phosphatase inhibitors during sample preparation to preserve phosphorylation status. A band at ~43–44 kDa is expected; slight mobility shifts may occur due to phosphorylation.
• For immunoprecipitation and ELISA, optimize antibody concentration empirically in the relevant sample system, considering the dynamic nature of ERK1 phosphorylation.
• In immunohistochemistry and immunocytochemistry, validate the phospho-specific signal by comparing treated/untreated samples or known activation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions one-stop access to high-quality IVD raw materials, including validated phospho-specific antibodies like this anti-ERK1-Y204 pAb. Whether you need optimized antibody pairs, bulk ancillary reagents, or assistance sourcing rare-target raw materials, our team provides tailored support to accelerate your assay development from concept to clinic. Partner with CamelBio for reliable supply and dedicated technical guidance in your MAPK pathway research.