Anti-Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb for WB, IF, ELISA - P28482 / P27361

Core Product Specifications and Parameters

Biological Background: ERK1/ERK2 Function and Localization

• MAPK1 (ERK2, p42-MAPK) and MAPK3 (ERK1, p44-MAPK) are serine/threonine kinases that function as the core of the MAPK/ERK signaling cascade, regulating cell growth, adhesion, survival, and differentiation through phosphorylation of over 160 substrates. Related references: PMID:35831023
• Phosphorylates phosphoglycerate kinase PGK1 under hypoxia, directing it to mitochondria to suppress acetyl-CoA production from pyruvate. Related references: PMID:26942675
• Localizes dynamically between the cytoplasm and nucleus, and is also found at the cytoskeleton, centrosome, focal adhesions, and caveolae, linking kinase activity to specific subcellular functions.
• Dual phosphorylation at Thr185/Tyr187 in ERK2 (and Thr202/Tyr204 in ERK1) is essential for catalytic activation; this antibody specifically recognizes the phosphorylated activation loop.
• Acts as a transcriptional repressor by directly binding a [GC]AAA[GC] DNA consensus sequence, independent of its kinase activity.
• Plays critical roles in receptor tyrosine kinase signaling (e.g., KIT), insulin signaling, and cell cycle progression, and is implicated in various cancers and developmental disorders.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide derived from the activation loop of human ERK2; expect cross-reactivity with the homologous phosphorylated region of ERK1 due to high sequence conservation.
• In WB, typical bands appear at ~42 kDa (phospho-ERK2) and ~44 kDa (phospho-ERK1) upon stimulation; confirm phospho-specificity by comparing with untreated or phosphatase-treated lysates.
• For IF/ICC, consider co-staining with a total ERK1/ERK2 antibody to validate activation status and subcellular translocation.
• Additional bands near 36–43 kDa may represent splice variants, post-translational modifications, or degradation products; validate in the relevant sample system.
• ELISA applications may be suitable for quantitative detection of phosphorylated ERK in cell or tissue lysates; optimize coating and detection conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive, one-stop sourcing platform for IVD raw materials. This phospho-ERK1/ERK2 antibody exemplifies our commitment to supporting MAPK/ERK signaling research with optimized, application-validated reagents. Our global supply network offers validated antibody pairs, bulk ancillary reagents, and rare-target raw material sourcing to help streamline your assay development from concept to clinic.