Anti-Phospho-ERK1 (T202) + ERK2 (T185) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P28482 / P27361

Core Product Specifications and Parameters

Biological Background: ERK1/ERK2 Function and Localization

• MAPK1 (ERK2) and MAPK3 (ERK1) are serine/threonine kinases that serve as essential components of the MAPK/ERK signal transduction cascade, regulating diverse cellular processes including growth, differentiation, and apoptosis.
• Upon activation, ERK1/2 phosphorylate over 160 substrates, including transcription factors (e.g., ATF2, ELK1, FOS), cytoskeletal elements, apoptosis regulators, and other kinases, thereby controlling gene expression and cellular dynamics (PubMed:35831023). Related references: PMID:35831023
• Under hypoxic conditions, ERK2 phosphorylates phosphoglycerate kinase PGK1 to promote its mitochondrial targeting and suppress acetyl-CoA formation (PubMed:26942675). Related references: PMID:26942675
• ERK2 localizes to the cytoplasm, nucleus, cytoskeleton (spindle, centrosome), membrane caveolae, and focal adhesions, reflecting its involvement in multiple subcellular signaling events.
• This kinase is regulated by phosphorylation on threonine and tyrosine residues within its activation loop (T185 for ERK2, T202 for ERK1) and is a phosphoprotein also subjected to acetylation and ubiquitination.
• ERK2 also acts as a transcriptional repressor by binding to GC-rich consensus sequences, inhibiting interferon gamma-induced gene expression independently of its kinase activity.
• The MAPK/ERK cascade is critical for cell cycle progression, mitosis, and meiosis, and its dysregulation is associated with various diseases, including cancer.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to the T202 region of human ERK1. Use phosphatase inhibitors during sample preparation to preserve the phosphorylation signal.
• For Western blotting, expected bands are observed at approximately 42 kDa (ERK2) and 44 kDa (ERK1). Optimize antibody dilution and incubation conditions for your specific sample.
• For immunohistochemistry (IHC-P), perform antigen retrieval and validate with positive control tissues expressing high levels of phospho-ERK1/2.
• This antibody is not suitable for detecting total ERK1/2 levels; use a pan-ERK antibody for total protein normalization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for the MAPK/ERK signaling research area, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. As your one-stop sourcing partner, we support diagnostic manufacturers and laboratories from concept to clinic with reliable supply and technical consulting.